Yin Biao, Zeng Yong, Wu Tonghua, Yu Shuyi, Xu Jian, Liu Su, Diao Lianghui, Zhao Zhenfu, Liang Desheng, Li Yuye
The State Key Laboratory of Medical Genetics of China, Central South University, Changsha, China.
Shenzhen Key Laboratory for Reproductive Immunology of Peri-implantation, Shenzhen Zhongshan Institute for Reproduction and Genetics, Fertility Center, Shenzhen Zhongshan Urology Hospital, Shenzhen, China.
Am J Reprod Immunol. 2017 Aug;78(2). doi: 10.1111/aji.12704. Epub 2017 May 15.
CD8 T cells are the main candidates to recognize and respond to fetal HLA-C at the fetal-maternal interface, but data on the amount of peripheral CD8 T cells and their functions during the window of implantation in recurrent implantation failure (RIF) patients are limited. Peripheral blood was obtained from 56 women with RIF and 16 fertile women in the mid-luteal phase of the menstrual cycle, and the CD8 T cells were determined by FACS analysis. No statistical differences in the proportion of peripheral CD8 T cells were observed among the women with RIF and the control group. However, the levels of IFN-γ and TNF-α CD8 T cells in the RIF group were significantly higher than those in the control group. The cytolytic activity and regulatory proportion of CD8 T cells in RIF were similar to that in the control group. Our data indicated that the elevated expression levels of IFN-γ and TNF-α in peripheral CD8 T cells may contribute to an impaired immune tolerance in women with RIF.
CD8 T细胞是在母胎界面识别并响应胎儿HLA - C的主要候选细胞，但关于复发性植入失败（RIF）患者植入窗口期外周血CD8 T细胞数量及其功能的数据有限。在月经周期的黄体中期，从56例RIF女性和16例有生育能力的女性中采集外周血，通过流式细胞术分析确定CD8 T细胞。RIF女性与对照组在外周血CD8 T细胞比例上未观察到统计学差异。然而，RIF组中IFN -γ和TNF -α CD8 T细胞水平显著高于对照组。RIF中CD8 T细胞的细胞溶解活性和调节比例与对照组相似。我们的数据表明，外周血CD8 T细胞中IFN -γ和TNF -α表达水平升高可能导致RIF女性免疫耐受受损。
