He Dafang, Ma Jideng, Long Keren, Wang Xun, Li Xuewei, Jiang Anan, Li Mingzhou
a Institute of Animal Genetics & Breeding, College of Animal Science & Technology , Sichuan Agricultural University , Wen'jiang , China.
Biosci Biotechnol Biochem. 2017 Aug;81(8):1478-1483. doi: 10.1080/09168451.2017.1322893. Epub 2017 May 16.
Glucose metabolism is a basic biological process that shows substantial variation within and between species. Using pig as a model organism, we investigated differences in glucose metabolic genes in seven tissues from domesticated pigs (Rongchang pig and Tibetan pig, meanwhile, the Tibetan pig just as a special case of the domesticated pig under plateau condition) and wild boar. We found large differences in the expression of genes involved in multiple aspects of glucose metabolism, including genes associated with glucose transport, gluconeogenesis, and glycolysis. In addition, we identified microRNAs (miRNAs) that may be involved in the divergence of glucose metabolism in pig. A combined analysis of mRNA and miRNA expression indicated that some miRNA:mRNA pairs showed ab facto function in it. Our results provide a valuable resource for further determination of miRNA regulatory roles in pig glucose metabolism and reveal the divergence of glucose metabolism in pigs under domestication.
葡萄糖代谢是一种基本的生物学过程，在物种内部和物种之间存在显著差异。我们以猪作为模式生物，研究了家猪（荣昌猪和藏猪，其中藏猪是高原条件下家猪的一个特殊例子）和野猪七个组织中葡萄糖代谢基因的差异。我们发现参与葡萄糖代谢多个方面的基因表达存在很大差异，包括与葡萄糖转运、糖异生和糖酵解相关的基因。此外，我们鉴定出了可能参与猪葡萄糖代谢差异的 microRNA（miRNA）。mRNA 和 miRNA 表达的联合分析表明，一些 miRNA:mRNA 对在其中发挥了实际功能。我们的结果为进一步确定 miRNA 在猪葡萄糖代谢中的调控作用提供了宝贵资源，并揭示了驯化条件下猪葡萄糖代谢的差异。
