Meng B Y, Tanaka M, Wakasugi T, Ohme M, Shinozaki K, Sugiura M
Center for Gene Research, Nagoya University, Japan.
Curr Genet. 1988 Oct;14(4):395-400. doi: 10.1007/BF00419998.
Transcription of the psaA operon in tobacco chloroplasts has been studied. This operon contains in linear sequence the genes encoding the P700 chlorophyll a A1 and A2 apoproteins (psaA and psaB) and the gene encoding the ribosomal CS14 protein (rps14). Northern blot hybridization revealed that a 5.2 kb transcript hybridizes to psaA, psaB, and rps14, but not to the fMet-tRNA (trnfM) gene which follows. Primer extension and in vitro capping assays indicated that the transcriptional initiation site is 194 bp upstream of psaA. The 3' end of the transcript was determined by S1 mapping to be 105 bp downstream of rps14. The transcript is calculated to be 5,207 nucleotides long.
对烟草叶绿体中psaA操纵子的转录进行了研究。该操纵子按线性顺序包含编码P700叶绿素a A1和A2脱辅基蛋白（psaA和psaB）的基因以及编码核糖体CS14蛋白（rps14）的基因。Northern印迹杂交显示，一个5.2 kb的转录本与psaA、psaB和rps14杂交，但不与随后的fMet - tRNA（trnfM）基因杂交。引物延伸和体外加帽试验表明，转录起始位点在psaA上游194 bp处。通过S1作图确定转录本的3'端在rps14下游105 bp处。计算得出该转录本长度为5207个核苷酸。
