Yu Yang, Zhang Xiaojun, Yuan Jianbo, Wang Quanchao, Li Shihao, Huang Hao, Li Fuhua, Xiang Jianhai
Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China.
University of Chinese Academy of Sciences, Beijing, 100049, China.
Mar Biotechnol (NY). 2017 Jun;19(3):277-286. doi: 10.1007/s10126-017-9749-5. Epub 2017 May 16.
The Pacific white shrimp Litopenaeus vannamei is a predominant aquaculture shrimp species in the world. Like other animals, the L. vannamei exhibited sexual dimorphism in growth trait. Mapping of the sex-determining locus will be very helpful to clarify the sex determination system and further benefit the shrimp aquaculture industry towards the production of mono-sex stocks. Based on the data used for high-density linkage map construction, linkage-mapping analysis was conducted. The sex determination region was mapped in linkage group (LG) 18. A large region from 0 to 21.205 cM in LG18 showed significant association with sex. However, none of the markers in this region showed complete association with sex in the other populations. So an association analysis was designed using the female parent, pool of female progenies, male parent, and pool of male progenies. Markers were de novo developed and those showing significant differences between female and male pools were identified. Among them, three sex-associated markers including one fully associated marker were identified. Integration of linkage and association analysis showed that the sex determination region was fine-mapped in a small region along LG18. The identified sex-associated marker can be used for the sex detection of this species at genetic level. The fine-mapped sex-determining region will contribute to the mapping of sex-determining gene and help to clarify sex determination system for L. vannamei.
南美白对虾凡纳滨对虾是世界上主要的养殖对虾品种。与其他动物一样，凡纳滨对虾在生长性状上表现出性别二态性。确定性别决定位点将有助于阐明性别决定系统，并进一步有利于对虾养殖业生产单性种群。基于用于构建高密度连锁图谱的数据，进行了连锁图谱分析。性别决定区域被定位在连锁群（LG）18上。LG18中从0到21.205厘摩的一个大区域与性别显示出显著关联。然而，该区域的标记在其他群体中均未显示出与性别完全关联。因此，设计了一项关联分析，使用雌性亲本、雌性后代池、雄性亲本和雄性后代池。从头开发标记，并鉴定出在雌性和雄性池之间存在显著差异的标记。其中，鉴定出三个与性别相关的标记，包括一个完全相关的标记。连锁和关联分析的整合表明，性别决定区域在LG18沿线的一个小区域内被精细定位。所鉴定的与性别相关的标记可用于在遗传水平上对该物种进行性别检测。精细定位的性别决定区域将有助于性别决定基因的定位，并有助于阐明凡纳滨对虾的性别决定系统。
