Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Sciences, Nanning, 530021, China.
Key Lab of Freshwater Animal Breeding, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Fishery, Huazhong Agriculture University, Wuhan, 430070, China.
BMC Genomics. 2020 Dec 2;21(1):857. doi: 10.1186/s12864-020-07254-x.
Ammonia is one of the most common toxicological environment factors affecting shrimp health. Although ammonia tolerance in shrimp is closely related to successful industrial production, few genetic studies of this trait are available.
In this study, we constructed a high-density genetic map of the Pacific white shrimp (Litopenaeus vannamei) using specific length amplified fragment sequencing (SLAF-seq). The constructed genetic map contained 17,338 polymorphic markers spanning 44 linkage groups, with a total distance of 6360.12 centimorgans (cM) and an average distance of 0.37 cM. Using this genetic map, we identified a quantitative trait locus (QTL) that explained 7.41-8.46% of the phenotypic variance in L. vannamei survival time under acute ammonia stress. We then sequenced the transcriptomes of the most ammonia-tolerant and the most ammonia-sensitive individuals from each of four genetically distinct L. vannamei families. We found that 7546 genes were differentially expressed between the ammonia-tolerant and ammonia-sensitive individuals. Using QTL analysis and the transcriptomes, we identified one candidate gene (annotated as an ATP synthase g subunit) associated with ammonia tolerance.
In this study, we constructed a high-density genetic map of L. vannamei and identified a QTL for ammonia tolerance. By combining QTL and transcriptome analyses, we identified a candidate gene associated with ammonia tolerance. Our work provides the basis for future genetic studies focused on molecular marker-assisted selective breeding.
氨是影响虾类健康的最常见的毒性环境因素之一。尽管虾类对氨的耐受性与成功的工业生产密切相关，但对这一特性的遗传研究却很少。
本研究利用特异长度扩增片段测序（SLAF-seq）构建了凡纳滨对虾（Litopenaeus vannamei）高密度遗传图谱。构建的遗传图谱包含 17338 个多态性标记，覆盖 44 个连锁群，总距离为 6360.12 厘摩（cM），平均距离为 0.37 cM。利用该遗传图谱，我们鉴定到一个数量性状位点（QTL），可解释凡纳滨对虾在急性氨胁迫下存活时间表型变异的 7.41-8.46%。随后，我们对来自四个遗传差异明显的凡纳滨对虾家系中每个最耐受氨和最敏感氨的个体进行了转录组测序。我们发现 7546 个基因在氨耐受个体和氨敏感个体之间存在差异表达。利用 QTL 分析和转录组数据，我们鉴定到一个与氨耐受相关的候选基因（注释为 ATP 合酶 g 亚基）。
本研究构建了凡纳滨对虾高密度遗传图谱，并鉴定到一个氨耐受 QTL。通过结合 QTL 和转录组分析，我们鉴定到一个与氨耐受相关的候选基因。我们的工作为未来集中于分子标记辅助选择育种的遗传研究提供了基础。
