Zhang Yaqun, Zhang Chuantao, Yao Na, Huang Jingxian, Sun Xiangshan, Zhao Bingran, Li Hengde
Chinese Academy of Fishery Sciences, Beijing, China.
Xiaying Enhancement and Experiment Station, Chinese Academy of Fishery Sciences, Weifang, Shandong, China.
PeerJ. 2021 Oct 29;9:e12390. doi: 10.7717/peerj.12390. eCollection 2021.
is one of the most important farmed shrimp species in many countries. Sexual dimorphism is observed in , in which females grow faster and larger than males; therefore, a unisexual female culture of could improve the efficiency of productivity. However, the genetic mechanisms underlying sex determination in are unclear. In this study, we constructed a high-density genetic linkage map of using genotyping-by-sequencing (GBS) technology in a full-sib family. The final map was 3,481.98 cM in length and contained 29,757 single nucleotide polymorphisms (SNPs). These SNPs were distributed on 41 sex-averaged linkage groups, with an average inter-marker distance of 0.123 cM. One haplotype, harboring five sex-specific SNPs, was detected in linkage group 1 (LG1), and its corresponding confidence interval ranged from 211.840 to 212.592 cM. Therefore, this high-density genetic linkage map will be informative for genome assembly and marker-assisted breeding, and the sex-linked SNPs will be helpful for further studies on molecular mechanisms of sex determination and unisexual culture of in the future.
是许多国家最重要的养殖虾类品种之一。在[虾的品种名称]中观察到了两性异形，其中雌性比雄性生长得更快、体型更大；因此，[虾的品种名称]的单性雌性养殖可以提高生产效率。然而，[虾的品种名称]性别决定的遗传机制尚不清楚。在本研究中，我们利用全同胞家系中的简化基因组测序（GBS）技术构建了[虾的品种名称]的高密度遗传连锁图谱。最终图谱长度为3481.98厘摩，包含29757个单核苷酸多态性（SNP）。这些SNP分布在41个平均分布性别的连锁群上，标记间平均距离为0.123厘摩。在连锁群1（LG1）中检测到一个单倍型，包含五个性别特异性SNP，其相应的置信区间为211.840至212.592厘摩。因此，这个高密度遗传连锁图谱将为基因组组装和标记辅助育种提供信息，并且与性别相关的SNP将有助于未来对[虾的品种名称]性别决定分子机制和单性养殖的进一步研究。
