Yoshioka K, Yoshida T, Wakabayashi Y, Nishioka H, Kondo M
First Department of Internal Medicine and Biochemistry, Kyoto Prefectural University of Medicine, Japan.
Endocrinol Jpn. 1988 Aug;35(4):537-43. doi: 10.1507/endocrj1954.35.537.
Brown adipose tissue (BAT) thermogenesis was assessed by measuring mitochondrial guanosine diphosphate (GDP) binding, cytochrome oxidase activity and oxygen consumption in ovariectomized (OVX) and sham-operated rats. The food intake and body weight of OVX rats increased more than those of controls and OVX rats became obese. Mitochondrial GDP binding, as an indicator of thermogenic activity, cytochrome oxidase activity, as a marker of mitochondrial abundance, and mitochondrial respiration of BAT in OVX rats were significantly reduced compared with those in controls. And, also, even when OVX rats were restricted in food intake (pair-gained) to produce comparable changes in body weight with sham-controls, or matched in food intake (pair-fed) with sham-controls, these parameters in both pair-gained and pair-fed OVX groups were decreased markedly compared to those in sham-controls. As expected, body weight in pair-fed OVX rats increased significantly more than that in sham-controls. In response to cold exposure, these parameters of OVX rats increased as much as those of controls did. These results suggest that reduced brown adipose tissue thermogenesis might be one of the important factors that are responsible for the development of obesity after OVX.
通过测量去卵巢（OVX）大鼠和假手术大鼠的线粒体鸟苷二磷酸（GDP）结合、细胞色素氧化酶活性和氧消耗来评估棕色脂肪组织（BAT）的产热情况。OVX大鼠的食物摄入量和体重增加幅度超过对照组，且OVX大鼠变得肥胖。与对照组相比，OVX大鼠作为产热活性指标的线粒体GDP结合、作为线粒体丰度标志物的细胞色素氧化酶活性以及BAT的线粒体呼吸均显著降低。而且，即使将OVX大鼠的食物摄入量限制（配对增重）以使其体重变化与假手术对照组相当，或者使其食物摄入量与假手术对照组匹配（配对喂养），配对增重和配对喂养的OVX组中的这些参数与假手术对照组相比仍显著降低。正如预期的那样，配对喂养的OVX大鼠体重增加显著超过假手术对照组。暴露于寒冷环境时，OVX大鼠的这些参数增加幅度与对照组相同。这些结果表明，棕色脂肪组织产热降低可能是OVX后肥胖发生的重要因素之一。
