人类红细胞中快速膜转运的核磁共振磁化转移分析。
NMR magnetization-transfer analysis of rapid membrane transport in human erythrocytes.
作者信息
Shishmarev Dmitry, Kuchel Philip W
机构信息
School of Life and Environmental Sciences, The University of Sydney, Sydney, NSW, 2006, Australia.
出版信息
Biophys Rev. 2016 Dec;8(4):369-384. doi: 10.1007/s12551-016-0221-y. Epub 2016 Oct 17.
Abstract
Nuclear magnetic resonance (NMR) magnetization-transfer (MT) experiments provide a convenient tool for studying rapid sub-second membrane-transport processes in situ in metabolically active cells. These experiments are used with membrane-permeable substances when separate (resolved) NMR signals are observed from their populations inside and outside the cells. Here, we provide a description of the theory and practice of the most common NMR MT experiments that have been used to study membrane-transport processes in human erythrocytes (red blood cells; RBCs). The procedures, involved in the analysis of the experimental data for defining mechanisms of transport, and for estimating values of kinetic parameters in the corresponding mathematical models, are given special attention.
摘要
核磁共振（NMR）磁化转移（MT）实验为原位研究代谢活跃细胞中快速的亚秒级膜运输过程提供了一种便捷工具。当从细胞内外的物质群体中观察到单独（分辨）的NMR信号时，这些实验用于可透过膜的物质。在此，我们描述了最常用于研究人类红细胞（红细胞；RBCs）膜运输过程的NMR MT实验的理论和实践。特别关注了分析实验数据以确定运输机制以及估计相应数学模型中动力学参数值所涉及的程序。
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