Woloszyk Anna, Mitsiadis Thimios A
Orofacial Development and Regeneration, Institute of Oral Biology, University of Zurich, Zurich, Switzerland.
Curr Protoc Stem Cell Biol. 2017 May 16;41:1F.19.1-1F.19.9. doi: 10.1002/cpsc.27.
The long-term survival and successful integration of implants for tissue replacement and regeneration highly depends upon the fast ingrowth of blood vessels from the surrounding tissues. Before selecting potential biomaterials for clinical applications, they must be thoroughly tested with proper analytical tools. This unit provides a protocol for studying the potential of cell-seeded scaffolds to attract vessels that will form vascular networks within biomaterials. It includes seeding of stem cells into silk fibroin scaffolds, angiogenesis assay on the chorioallantoic membrane (CAM) of fertilized chicken eggs, a procedure for perfusion with MicroFil, and finally microcomputed tomography (µCT) scanning. This technique can help screen potential biomaterial implants, thereby reducing the amount of animals needed for pre-clinical in vivo studies. © 2017 by John Wiley & Sons, Inc.
用于组织替代和再生的植入物的长期存活及成功整合在很大程度上取决于周围组织血管的快速长入。在选择用于临床应用的潜在生物材料之前，必须使用适当的分析工具对其进行全面测试。本单元提供了一个方案，用于研究接种细胞的支架吸引血管并在生物材料内形成血管网络的潜力。它包括将干细胞接种到丝素蛋白支架中、在受精鸡蛋的绒毛尿囊膜（CAM）上进行血管生成测定、用MicroFil灌注的步骤，以及最后进行微计算机断层扫描（µCT）扫描。该技术有助于筛选潜在的生物材料植入物，从而减少临床前体内研究所需的动物数量。© 2017约翰威立国际出版公司。
