插入序列IS5在其末端含有一个急剧弯曲的DNA结构。
Insertion sequence IS5 contains a sharply curved DNA structure at its terminus.
作者信息
Muramatsu S, Kato M, Kohara Y, Mizuno T
机构信息
Laboratory of Microbiology, School of Agriculture, Nagoya University, Japan.
出版信息
Mol Gen Genet. 1988 Nov;214(3):433-8. doi: 10.1007/BF00330477.
It was demonstrated that insertion sequence IS5 contains a sequence-directed bent (sharply curved) DNA structure at its terminus, close to one of its 16 bp terminal repeats. The minimal number of copies of IS5 related sequences and the locations of the latter on the Escherichia coli K12 W3110 chromosome were determined. Evidence is presented of the occurrence of IS5 mediated translocation and duplication of a large DNA segment on the E. coli chromosome.
已证明插入序列IS5在其末端靠近16bp末端重复序列之一处含有一个序列导向的弯曲（急剧弯曲）DNA结构。确定了IS5相关序列的最小拷贝数及其在大肠杆菌K12 W3110染色体上的位置。提供了关于IS5介导大肠杆菌染色体上一个大DNA片段易位和重复发生的证据。
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