DNA聚合酶III的校对亚基ε与参与大肠杆菌SOS应答的蛋白质之间的相互作用。
Interactions between epsilon, the proofreading subunit of DNA polymerase III, and proteins involved in the SOS response of Escherichia coli.
作者信息
Foster P L, Sullivan A D
机构信息
Division of Environmental Health, Boston University School of Public Health, MA.
出版信息
Mol Gen Genet. 1988 Nov;214(3):467-73. doi: 10.1007/BF00330482.
Abstract
Epsilon, a fidelity subunit of Escherichia coli DNA Polymerase III, is encoded by dnaQ+. dnaQ49 is a recessive allele that confers temperature-sensitive and salt-suppressible phenotypes for both replication fidelity and viability. SOS mutagenesis in E. coli is regulated by LexA and requires activated RecA (RecA*) and the products of the umuDC operon. dnaQ49 strains with various recA, lexA and umuDC alleles were constructed to determine if activities induced as part of the SOS response influence epsilon activity. We found: (1) both UmuDC and RecA* independently enhance the dnaQ49 mutator phenotype, and (2) expression of RecA* activity in the absence of UmuDC function increases the temperature sensitivity for viability of dnaQ49. These results support the hypothesis that RecA and one or both of the UmuDC proteins interact with the replication complex during SOS mutagenesis.
摘要
ε是大肠杆菌DNA聚合酶III的一个保真亚基，由dnaQ⁺编码。dnaQ49是一个隐性等位基因，它赋予复制保真度和生存力温度敏感和盐抑制表型。大肠杆菌中的SOS诱变由LexA调控，需要激活的RecA（RecA*）和umuDC操纵子的产物。构建了具有各种recA、lexA和umuDC等位基因的dnaQ49菌株，以确定作为SOS反应一部分诱导的活性是否影响ε活性。我们发现：（1）UmuDC和RecA都独立增强dnaQ49的诱变表型，（2）在没有UmuDC功能的情况下RecA活性的表达增加了dnaQ49生存力的温度敏感性。这些结果支持了RecA以及UmuDC蛋白中的一个或两个在SOS诱变期间与复制复合物相互作用的假设。
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