Verma Shailza, Saxena Rahul, Siddiqui Merajul Haque, Santha Karunathy, Sethupathy Subramaniam
Assistant Professor, Department of Biochemistry, School of Medical Sciences and Research, Sharda University, Greater Noida, Uttar Pradesh, India.
Assistant Professor, Department of Biochemistry, School of Allied Health Sciences, Sharda University, Greater Noida, Uttar Pradesh, India.
J Clin Diagn Res. 2017 Mar;11(3):BC01-BC05. doi: 10.7860/JCDR/2017/22196.9324. Epub 2017 Mar 1.
Oral Squamous Cell Carcinoma (OSCC) affects global health with increasing incidence and mortality rate. It may involve exposure to carcinogens from tobacco smoking or chewing. Alteration in expression of gene encoding the enzymes concerned with carcinogen biotransformation along with oxidative stress may increase or decrease the risk of cancer.
To evaluate the expression of gene in OSCC patients along with its relation with oxidative stress and phase 2 detoxification enzyme status.
In the present study, CYP1B1 genotypic analysis was carried out along with estimation of serum Total Antioxidant Activity (TAA), erythrocyte Malondialdehyde (MDA) levels and serum Glutathione-S-Transferase (GST) activity in 20 OSCC patients and statistically compared with that of age matched 20 healthy subjects, served as control by using student's t-test.
It was observed that 85% of histopathologically diagnosed OSCC patients had expression with significantly elevated levels of MDA (p<0.001). In addition, plasma total antioxidant status and serum GST levels were decreased significantly (p<0.05) in OSCC patients as compared to the healthy controls to overcome the burden of oxidative stress.
On the basis of the present study, we conclude that the expression of CYP1B1 is an important determinant of carcinogenesis and significantly associated with oxidative stress characterized by decreased serum GST and total antioxidant levels in OSCC patients.
口腔鳞状细胞癌（OSCC）发病率和死亡率不断上升，影响着全球健康。它可能与接触吸烟或咀嚼烟草中的致癌物有关。与致癌物生物转化相关的酶编码基因表达的改变以及氧化应激可能增加或降低癌症风险。
评估OSCC患者中基因的表达及其与氧化应激和二期解毒酶状态的关系。
在本研究中，对20例OSCC患者进行了CYP1B1基因分型分析，并测定了血清总抗氧化活性（TAA）、红细胞丙二醛（MDA）水平和血清谷胱甘肽-S-转移酶（GST）活性，并与20名年龄匹配的健康受试者进行统计学比较，以学生t检验作为对照。
观察到85%经组织病理学诊断的OSCC患者有表达，MDA水平显著升高（p<0.001）。此外，与健康对照组相比，OSCC患者血浆总抗氧化状态和血清GST水平显著降低（p<0.05），以克服氧化应激负担。
基于本研究，我们得出结论，CYP1B1的表达是致癌作用的重要决定因素，并且与OSCC患者血清GST降低和总抗氧化水平降低所表征的氧化应激显著相关。
