Department of Pharmaceutics, AISSMS College of Pharmacy, Near RTO, Kennedy Road, Pune 411001, Maharashtra, India.
Department of Pharmaceutics, AISSMS College of Pharmacy, Near RTO, Kennedy Road, Pune 411001, Maharashtra, India.
Int J Biol Macromol. 2017 Oct;103:338-346. doi: 10.1016/j.ijbiomac.2017.05.031. Epub 2017 May 13.
Psyllium seed polysaccharide was modified to investigate its use as multifunctional pharmaceutical excipient. The objective of this study was isolation of psyllium seed polysaccharide and crosslinking with acrylic acid using N,N-methylene bisacrylamide and its characterization. Acrylic acid was used as monomer and ammonium persulfate as initiator. A full factorial design was employed to optimize the crosslinking. The modified polysaccharide was characterized by FTIR, DSC, PXRD, loss on drying, pH, viscosity, micromeritics properties and swelling studies in 0.1N HCl, 0.5N NaOH, phosphate buffer pH 6.8. It was observed that swelling of crosslinked polysaccharide increased with decreased concentration of monomer and increasing concentration of crosslinker. Greater degree of grafting was observed with increase in crosslinker and monomer concentration. Dispersions of 1% w/v of PPS and APPS show pseudoplastic behavior. No clinical signs of toxicity were evident in repeat dose toxicity studies conducted in rats. Administration of up to 350mg/kg/day of APPS was well tolerated by the animals. Modification of psyllium via graft copolymerization and network formation with the crosslinker, improved the property profile and utility of psyllium polysaccharide. The modified polysaccharide can be used for designing controlled release drug delivery systems due to its swelling ability.
车前子种多糖被修饰以研究其作为多功能药物赋形剂的用途。本研究的目的是分离车前子种多糖，并与丙烯酰胺使用 N,N-亚甲基双丙烯酰胺进行交联及其特性。使用丙烯酰胺作为单体和过硫酸铵作为引发剂。采用全因子设计来优化交联。用 FTIR、DSC、PXRD、干燥失重、pH 值、粘度、微尺度性质和在 0.1N HCl、0.5N NaOH、pH6.8 的磷酸盐缓冲液中的溶胀研究对修饰后的多糖进行了表征。结果表明，随着单体浓度的降低和交联剂浓度的增加，交联多糖的溶胀增加。随着交联剂和单体浓度的增加，接枝程度增大。在 1%w/v 的 PPS 和 APPS 分散体中表现出假塑性行为。在对大鼠进行的重复剂量毒性研究中，没有明显的毒性临床迹象。动物耐受高达 350mg/kg/天的 APPS 给药。通过与交联剂的接枝共聚和网络形成对车前子多糖进行修饰，改善了车前子多糖的性能特征和用途。由于其溶胀能力，修饰后的多糖可用于设计控制释放药物传递系统。
