Vieira Paulo, Mowery Joseph, Kilcrease James, Eisenback Jonathan D, Kamo Kathryn
Department of Plant Pathology, Physiology, and Weed Science, Virginia Tech, Blacksburg, VA 24061.
Floral and Nursery Plants Research Unit, U.S. National Arboretum, U.S. Department of Agriculture, Beltsville, MD 20705-2350.
J Nematol. 2017 Mar;49(1):2-11. doi: 10.21307/jofnem-2017-040.
cv. Nellie White, commonly known as Easter lily, is an important floral crop with an annual wholesale value of over $26 million in the United States. The root-lesion nematode, , is a major pest of lily due to the significant root damage it causes. In this study, we investigated the cytological aspects of this plant-nematode interaction using bright-field and transmission electron microscopy. We took advantage of an in vitro culture method to multiply lilies and follow the nematode infection over time. Phenotypic reactions of roots inoculated with were evaluated from 0 to 60 d after nematode infection. Symptom development progressed from initial randomly distributed discrete necrotic areas to advanced necrosis along entire roots of each inoculated plant. A major feature characterizing this susceptible host response to nematode infection was the formation of necrosis, browning, and tissue death involving both root epidermis and cortical cells. Degradation of consecutive cell walls resulted in loss of cell pressure, lack of cytoplasmic integrity, followed by cell death along the intracellular path of the nematode's migration. was never seen in the vascular cylinder as the layer of collapsed endodermal cells presumably blocked the progression of nematodes into this area of the roots. This study presents the first detailed cytological characterization of infection of Easter lily plants.
cv. 内莉·怀特，通常被称为复活节百合，是一种重要的花卉作物，在美国年批发价值超过2600万美元。根结线虫是百合的主要害虫，因为它会造成严重的根系损害。在本研究中，我们使用明场和透射电子显微镜研究了这种植物与线虫相互作用的细胞学方面。我们利用体外培养方法繁殖百合，并随时间跟踪线虫感染情况。在接种线虫后0至60天评估接种线虫的根的表型反应。症状发展从最初随机分布的离散坏死区域发展到每个接种植物整个根系的晚期坏死。这种易感宿主对线虫感染反应的一个主要特征是形成坏死、褐变以及涉及根表皮和皮层细胞的组织死亡。连续细胞壁的降解导致细胞压力丧失、细胞质完整性缺失，随后沿线虫迁移的细胞内路径细胞死亡。在维管束中从未见过线虫，因为内皮层细胞层塌陷可能阻止了线虫进入根系的这个区域。本研究首次详细描述了复活节百合植株受线虫感染的细胞学特征。
