In vitro screening for protein tyrosine phosphatase 1B and dipeptidyl peptidase IV inhibitors from selected Nigerian medicinal plants.

BACKGROUND/AIM: Protein tyrosine phosphatase 1B (PTP 1B) and dipeptidyl peptidase IV (DPP IV) have been identified as one of the drug targets for the treatment of Type-2 diabetes. This study was designed to screen for PTP 1B and DPP-IV inhibitors from some Nigerian medicinal plants.

MATERIALS AND METHODS

PTP 1B and DPP-IV drug discovery kits from Enzo Life Sciences were used to investigate in vitro inhibitory effect of crude methanolic extract of 10 plants; , , , , , , , , , and .

RESULTS

The results indicated PTP IB inhibition by (68.2 ± 2.29%), (67.4 ± 2.80%), (57.2 ± 2.50%), (55.1 ± 2.19%), and (41.2 ± 1.87%) were significantly ( < 0.05) higher as compared with standard inhibitor, sumarin while that of (18.1 ± 2.00%) was significantly lower as compared with sumarin. The PTB 1B inhibition by (31.5 ± 1.90%) was not significantly ( > 0.05) different from that of sumarin. The DPP-IV inhibition by (68.1 ± 2.71%) was significantly higher as compared with a known inhibitor, P32/98. (57.0±1.91%), (56.6±2.01%), (51.0±1.30%), (44.6 ± 2.40%), (36.2 ± 2.00%), (35.4 ± 2.10%), and (33.6 ± 1.50%) show significantly ( < 0.05) lower inhibitions toward DPP-IV.

CONCLUSION

The work demonstrated that these plant materials could serve as sources of lead compounds in the development of anti-diabetic agent(s) targeting PTP 1B and/or DPP-IV.