Pant Dipak Raj, Pant Narayan Dutt, Saru Dil Bahadur, Yadav Uday Narayan, Khanal Dharma Prasad
Department of Pharmacy, National Model College for Advance Learning, Kathmandu, Nepal.
Department of Microbiology, Grande International Hospital, Dhapasi, Kathmandu, Nepal.
J Intercult Ethnopharmacol. 2017 Apr 12;6(2):170-176. doi: 10.5455/jice.20170403094055. eCollection 2017 Apr-Jun.
The main aims of the study were to evaluate the phytochemical constituents and to study the antioxidant, antimicrobial, antidiabetic, anti-inflammatory, and analgesic activities of extracts from stem wood of .
Ethanol, acetone and isopropyl alcohol (IPA) (1:1) extracts of stem wood of were subjected to phytochemical screening and analysis of biological activities from August 2015 to January 2016. The antioxidant assay was carried out using 2, 2-diphenyl-1-picrylhydrazyl scavenging method, antimicrobial activity testing by cup diffusion method, antidiabetic test evaluation by oral glucose tolerance test in mice, anti-inflammatory effect was evaluated by hind paw edema method in mice and analgesic test evaluation by a chemical writhing method in mice.
The results of the study revealed that is a source of various phytoconstituents such as alkaloids, glycosides, saponins, tannins, proteins, carbohydrates, cardiac glycosides, flavonoids, and terpenoids. Both the acetone and IPA extract as well as the ethanol extract of stem wood of exhibited a dose-dependent antioxidant activity. Acetone and IPA extract showed antibacterial activity against Gram-positive bacteria, while the ethanolic extract was found to possess antidiabetic activity. The antidiabetic activity of the extract was found to be time and dose-dependent. Similarly, the acetone and IPA extract was found to have anti-inflammatory activity, which was also time and dose-dependent. Furthermore, the ethanolic extract showed analgesic activity, which was dose-dependent. The ethanolic extract was found to be nontoxic.
Thus, this study laid sufficient background for the further research on extracts from stem wood of for identification, subsequent purification and isolation of compounds having antibacterial, antidiabetic, anti-inflammatory, and analgesic activities.
本研究的主要目的是评估植物化学成分，并研究[植物名称]茎木提取物的抗氧化、抗菌、抗糖尿病、抗炎和镇痛活性。
2015年8月至2016年1月，对[植物名称]茎木的乙醇、丙酮和异丙醇（IPA）（1:1）提取物进行植物化学筛选和生物活性分析。抗氧化测定采用2,2-二苯基-1-苦基肼自由基清除法，抗菌活性测试采用杯碟扩散法，抗糖尿病测试通过小鼠口服葡萄糖耐量试验进行评估，抗炎作用通过小鼠后爪肿胀法进行评估，镇痛测试通过小鼠化学扭体法进行评估。
研究结果表明，[植物名称]是多种植物成分的来源，如生物碱、糖苷、皂苷、单宁、蛋白质、碳水化合物、强心苷、黄酮类化合物和萜类化合物。[植物名称]茎木的丙酮提取物、IPA提取物以及乙醇提取物均表现出剂量依赖性的抗氧化活性。丙酮提取物和IPA提取物对革兰氏阳性菌具有抗菌活性，而乙醇提取物具有抗糖尿病活性。提取物的抗糖尿病活性具有时间和剂量依赖性。同样，丙酮提取物和IPA提取物具有抗炎活性，也具有时间和剂量依赖性。此外，乙醇提取物表现出镇痛活性，具有剂量依赖性。乙醇提取物无毒。
因此，本研究为进一步研究[植物名称]茎木提取物以鉴定、随后纯化和分离具有抗菌、抗糖尿病、抗炎和镇痛活性的化合物奠定了充分的基础。
