环孢素A对小鼠实验性沙门氏菌病的影响。

Effect of cyclosporin A on murine experimental salmonellosis.

作者信息

Muthukkumar S, Muthukkaruppan V

机构信息

Department of Immunology, School of Biological Sciences, Madurai Kamaraj University, India.

出版信息

Comp Immunol Microbiol Infect Dis. 1988;11(3-4):153-61. doi: 10.1016/0147-9571(88)90032-x.

DOI:10.1016/0147-9571(88)90032-x

PMID:2851407

Abstract

Investigations were undertaken to evaluate the effect of cyclosporin A (CyA) on primary infection of mice with Salmonella typhimurium. Further, its effect on delayed-type hypersensitivity (DTH) and antibody response to porin, an outer membrane protein of S. typhimurium, has been characterized under different CyA regimen. When mice were infected i.p. with 5 x 10(4) live medium virulent organisms, the bacterial growth increased by about 2 log10 in CyA treated mice. CyA administered daily for 2 weeks following immunization with porin caused profound suppression of DTH and both IgM and IgG antibody responses. Given a single dose of CyA 24 h before DTH elicitation, the drug had a marked suppressive effect on DTH, but not on the antibody response to porin. Treatment on days 0-5 following immunization had no effect on the manifestation of the DTH response nor on the anti-porin IgM response whereas an enhanced level of IgG was observed in this group.

摘要

开展了多项研究以评估环孢素A（CyA）对鼠伤寒沙门氏菌原发性感染小鼠的影响。此外，还在不同的CyA给药方案下，对其在迟发型超敏反应（DTH）以及对鼠伤寒沙门氏菌外膜蛋白孔蛋白的抗体反应方面的作用进行了表征。当小鼠经腹腔注射5×10⁴个活的中等毒力菌时，在接受CyA治疗的小鼠中细菌生长增加了约2个对数级。在用孔蛋白免疫后每天给予CyA持续2周，会导致DTH以及IgM和IgG抗体反应受到显著抑制。在引发DTH前24小时给予单剂量的CyA，该药物对DTH有显著抑制作用，但对孔蛋白的抗体反应无影响。在免疫后第0 - 5天进行治疗，对DTH反应的表现以及抗孔蛋白IgM反应均无影响，而在该组中观察到IgG水平升高。