Klop Anthony C, Vester Marloes E M, Colman Kerri L, Ruijter Jan M, Van Rijn Rick R, Oostra Roelof-Jan
Department of Anatomy, Embryology and Physiology, Academic Medical Center, University of Amsterdam, 1105, AZ Amsterdam, The Netherlands.
Department of Radiology, Academic Medical Center, University of Amsterdam, 1105, AZ Amsterdam, The Netherlands.
Clin Anat. 2017 Sep;30(6):799-804. doi: 10.1002/ca.22917. Epub 2017 Jun 19.
The aim of this study was to determine whether effects of repetitive freeze-thaw cycles, with various thawing temperatures, on human muscle tissue can be quantified using postmortem computed tomography (PMCT) technology. An additional objective was to determine the preferred thawing temperature for muscle tissue in this study. Human cadaver upper extremities were divided into two different thawing temperature groups and underwent a series of four freeze-thaw cycles in total. Axial CT scans were performed after each cycle. CT attenuation (in Hounsfield units, HU) was measured in four muscles of the upper extremities. HU values changed significantly with the introduction of each subsequent freeze-thaw cycle. Moreover, the changes in HU values were different for each thawing group. There was a significant increase of HU values in both groups between t and t . Unfrozen tissue showed large variation of HU values in all samples. It was possible to distinguish between samples thawed at different thawing temperatures based on their respective HU values. It is advisable to keep the number of freeze-thaw cycles to just one, if the human cadaveric tissue is to be used for educational purposes. The preferred thawing temperature in this study is 2°C. Clin. Anat. 30:799-804, 2017. © 2017Wiley Periodicals, Inc.
本研究的目的是确定使用死后计算机断层扫描（PMCT）技术能否量化不同解冻温度下重复冻融循环对人体肌肉组织的影响。另一个目标是确定本研究中肌肉组织的最佳解冻温度。将人类尸体上肢分为两个不同的解冻温度组，总共进行一系列四个冻融循环。每个循环后进行轴向CT扫描。在上肢的四块肌肉中测量CT衰减（以亨氏单位，HU表示）。随着每个后续冻融循环的引入，HU值发生了显著变化。此外，每个解冻组的HU值变化不同。在t和t之间，两组的HU值均显著增加。未冷冻组织在所有样本中显示出较大的HU值变化。根据各自的HU值，可以区分在不同解冻温度下解冻的样本。如果将人体尸体组织用于教育目的，建议将冻融循环次数仅保持为一次。本研究中的最佳解冻温度为2°C。《临床解剖学》30:799 - 804，2017年。©2017威利期刊公司。
