生能系统评估生酮饮食对肝脏的影响。
A bioenergetics systems evaluation of ketogenic diet liver effects.
机构信息
a Kansas City University of Medicine and Biosciences, Kansas City, MO 64106, USA.
b University of Kansas Alzheimer's Disease Center, University of Kansas Medical Center, Kansas City, KS 66160, USA.
出版信息
Appl Physiol Nutr Metab. 2017 Sep;42(9):955-962. doi: 10.1139/apnm-2017-0068. Epub 2017 May 17.
Abstract
Ketogenic diets induce hepatocyte fatty acid oxidation and ketone body production. To further evaluate how ketogenic diets affect hepatocyte bioenergetic infrastructure, we analyzed livers from C57Bl/6J male mice maintained for 1 month on a ketogenic or standard chow diet. Compared with the standard diet, the ketogenic diet increased cytosolic and mitochondrial protein acetylation and also altered protein succinylation patterns. SIRT3 protein decreased while SIRT5 protein increased, and gluconeogenesis, oxidative phosphorylation, and mitochondrial biogenesis pathway proteins were variably and likely strategically altered. The pattern of changes observed can be used to inform a broader systems overview of how ketogenic diets affect liver bioenergetics.
摘要
生酮饮食可诱导肝细胞脂肪酸氧化和酮体生成。为了进一步评估生酮饮食如何影响肝细胞的生物能量基础设施，我们分析了维持 1 个月生酮饮食或标准饲料饮食的 C57Bl/6J 雄性小鼠的肝脏。与标准饮食相比，生酮饮食增加了细胞质和线粒体蛋白乙酰化，并改变了蛋白琥珀酰化模式。SIRT3 蛋白减少，而 SIRT5 蛋白增加，糖异生、氧化磷酸化和线粒体生物发生途径的蛋白质也发生了不同程度的变化，可能是策略性的改变。观察到的变化模式可用于更全面地了解生酮饮食如何影响肝脏的生物能量学。
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Cell Metab. 2017 Feb 7;25(2):262-284. doi: 10.1016/j.cmet.2016.12.022.
2
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Am J Physiol Regul Integr Comp Physiol. 2016 Aug 1;311(2):R337-51. doi: 10.1152/ajpregu.00156.2016. Epub 2016 Jun 29.
3
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Biochim Biophys Acta. 2015 Oct;1852(10 Pt A):2056-65. doi: 10.1016/j.bbadis.2015.07.009. Epub 2015 Jul 11.
4
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Epilepsia. 2015 Jul;56(7):e95-8. doi: 10.1111/epi.13038. Epub 2015 May 23.
5
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J Bioenerg Biomembr. 2015 Apr;47(1-2):1-11. doi: 10.1007/s10863-014-9570-z. Epub 2014 Aug 8.
6
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Cell Metab. 2013 Dec 3;18(6):920-33. doi: 10.1016/j.cmet.2013.11.013.
7
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Trends Endocrinol Metab. 2014 Jan;25(1):42-52. doi: 10.1016/j.tem.2013.09.002. Epub 2013 Oct 18.
8
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Br J Pharmacol. 2014 Apr;171(8):1854-69. doi: 10.1111/bph.12394.
9
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PLoS One. 2013 Jul 9;8(7):e69330. doi: 10.1371/journal.pone.0069330. Print 2013.
10
SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways.SIRT5 介导的赖氨酸去琥珀酰化作用影响多种代谢途径。
Mol Cell. 2013 Jun 27;50(6):919-30. doi: 10.1016/j.molcel.2013.06.001.
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