利用酵母中的Ty元件进行转座子标签法。
Transposon tagging using Ty elements in yeast.
作者信息
Garfinkel D J, Mastrangelo M F, Sanders N J, Shafer B K, Strathern J N
机构信息
Bionetics Research, Inc., National Cancer Institute-Frederick Cancer Research Facility, Maryland 21701.
出版信息
Genetics. 1988 Sep;120(1):95-108. doi: 10.1093/genetics/120.1.95.
Abstract
We have used the ability to induce high levels of Ty transposition to develop a method for transposon mutagenesis in Saccharomyces cerevisiae. To facilitate genetic and molecular analysis, we have constructed GAL1-promoted TyH3 or Ty917 elements that contain unique cloning sites, and marked these elements with selectable genes. These genes include the yeast HIS3 gene, and the plasmid PiAN7 containing the Tn903 NEO gene. The marked Ty elements retain their ability to transpose, to mutate the LYS2, LYS5, or STE2 genes, and to activate the promoterless his3 delta 4 target gene. Ty elements containing selectable genes are also useful in strain construction, in chromosomal mapping, and in gene cloning strategies.
摘要
我们利用诱导高水平Ty转座的能力，开发了一种在酿酒酵母中进行转座子诱变的方法。为便于进行遗传和分子分析，我们构建了含有独特克隆位点的GAL1启动的TyH3或Ty917元件，并用选择基因对这些元件进行标记。这些基因包括酵母HIS3基因，以及含有Tn903 NEO基因的质粒PiAN7。标记后的Ty元件保留了其转座能力、使LYS2、LYS5或STE2基因突变的能力，以及激活无启动子his3 delta 4靶基因的能力。含有选择基因的Ty元件在菌株构建、染色体定位和基因克隆策略中也很有用。
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本文引用的文献
1
Temperature effects on the rate of ty transposition.温度对转座酶切的影响。
Science. 1984 Oct 5;226(4670):53-5. doi: 10.1126/science.226.4670.53.
2
Selection of lys2 Mutants of the Yeast SACCHAROMYCES CEREVISIAE by the Utilization of alpha-AMINOADIPATE.利用 α-氨基己二酸筛选酵母 SACCHAROMYCES CEREVISIAE 的 lys2 突变株。
Genetics. 1979 Sep;93(1):51-65. doi: 10.1093/genetics/93.1.51.
3
Nucleotide sequences of STE2 and STE3, cell type-specific sterile genes from Saccharomyces cerevisiae.STE2 和 STE3 的核苷酸序列，酿酒酵母中细胞类型特异性的不育基因。
EMBO J. 1985 Oct;4(10):2643-8. doi: 10.1002/j.1460-2075.1985.tb03982.x.
4
Sequence variation in dispersed repetitive sequences in Saccharomyces cerevisiae.酿酒酵母中分散重复序列的序列变异。
J Mol Biol. 1981 Feb 5;145(4):619-32. doi: 10.1016/0022-2836(81)90306-5.
5
Mutants of Saccharomyces cerevisiae unresponsive to cell division control by polypeptide mating hormone.对多肽交配激素的细胞分裂控制无反应的酿酒酵母突变体。
J Cell Biol. 1980 Jun;85(3):811-22. doi: 10.1083/jcb.85.3.811.
6
Purification of genomic sequences from bacteriophage libraries by recombination and selection in vivo.通过体内重组和筛选从噬菌体文库中纯化基因组序列。
Nucleic Acids Res. 1983 Apr 25;11(8):2427-45. doi: 10.1093/nar/11.8.2427.
7
A positive selection for mutants lacking orotidine-5'-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance.酵母中缺乏乳清苷-5'-磷酸脱羧酶活性的突变体的正向选择：5-氟乳清酸抗性。
Mol Gen Genet. 1984;197(2):345-6. doi: 10.1007/BF00330984.
8
Binding of alpha-factor pheromone to yeast a cells: chemical and genetic evidence for an alpha-factor receptor.α-因子信息素与酵母a细胞的结合：α-因子受体的化学和遗传学证据。
Cell. 1983 Dec;35(2 Pt 1):521-9. doi: 10.1016/0092-8674(83)90186-1.
9
Transformation of intact yeast cells treated with alkali cations.经碱金属阳离子处理的完整酵母细胞的转化
J Bacteriol. 1983 Jan;153(1):163-8. doi: 10.1128/jb.153.1.163-168.1983.
10
Mutations affecting Ty-mediated expression of the HIS4 gene of Saccharomyces cerevisiae.影响酿酒酵母HIS4基因Ty介导表达的突变。
Genetics. 1984 Jun;107(2):179-97. doi: 10.1093/genetics/107.2.179.
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