Leroy Claire, Tobin Kari Anne Risan, Basak Sanjay, Cathrine Staff Anne, Duttaroy Asim K
Department of Nutrition, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, Oslo, Norway.
Department of Nutrition, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, Oslo, Norway; Molecular Biology Division, National Institute Nutrition, Indian Council of Medical Research, Hyderabad, India.
Prostaglandins Leukot Essent Fatty Acids. 2017 May;120:1-7. doi: 10.1016/j.plefa.2017.04.002. Epub 2017 Apr 5.
Cellular uptake of long chain fatty acids in human placental trophoblasts is thought to be mediated by several membrane- and cytoplasmic fatty acid-binding proteins (FABP). FABP3 was shown to be involved in long chain polyunsaturated fatty acids (LCPUFA) uptake in human trophoblastic choriocarcinoma cells, BeWo as the uptake of arachidonic acid,20:4n-6 (ARA) was decreased in FABP3-knockdown BeWo cells. However, the regulation of expression of FABP3 in these cells is not yet well known. The aim of the present study was to examine the FABP3 expression by LCPUFAs, insulin and LXR agonists in BeWo cells. Among all these fatty acids tested, only ARA dose-dependently stimulated the expression of FABP3 protein in these cells after 24h incubation while other fatty acids had no such effect. In addition, LXR agonist and insulin dose-dependently increased FABP3 protein expression in these cells after 24h incubation. Insulin-stimulated FABP3 protein expression was accompanied with an increased arachidonic acid uptake. Differentiated BeWo cells had lesser expression of FABP3 protein than in the undifferentiated cells as the cellular differentiation state was measured by hCG production. In preeclamptic placental tissue, lowered expression of FABP3 protein was observed compared with those in normal pregnancy. All these data indicate that FABP3 may in be part involved in ARA uptake in these cells and its expression may be regulated by ARA, insulin, LXR and the state of cellular differentiation.
人们认为，人胎盘滋养层细胞对长链脂肪酸的摄取是由几种膜脂肪酸结合蛋白和细胞质脂肪酸结合蛋白（FABP）介导的。FABP3已被证明参与人滋养层绒癌BeWo细胞对长链多不饱和脂肪酸（LCPUFA）的摄取，因为在FABP3基因敲低的BeWo细胞中，花生四烯酸（20:4n-6，ARA）的摄取减少。然而，这些细胞中FABP3表达的调控机制尚未完全明确。本研究的目的是检测LCPUFA、胰岛素和LXR激动剂对BeWo细胞中FABP3表达的影响。在所有测试的脂肪酸中，只有ARA在孵育24小时后能剂量依赖性地刺激这些细胞中FABP3蛋白的表达，而其他脂肪酸则无此作用。此外，LXR激动剂和胰岛素在孵育24小时后能剂量依赖性地增加这些细胞中FABP3蛋白的表达。胰岛素刺激FABP3蛋白表达的同时，花生四烯酸的摄取也增加。通过检测hCG的分泌来衡量细胞分化状态，结果显示，分化后的BeWo细胞中FABP3蛋白的表达低于未分化细胞。与正常妊娠胎盘组织相比，子痫前期胎盘组织中FABP3蛋白的表达降低。所有这些数据表明，FABP3可能部分参与了这些细胞对ARA的摄取，其表达可能受ARA、胰岛素、LXR和细胞分化状态的调控。
