Drumm M L, Smith C L, Dean M, Cole J L, Iannuzzi M C, Collins F S
Department of Internal Medicine, University of Michigan, Ann Arbor 48109.
Genomics. 1988 May;2(4):346-54. doi: 10.1016/0888-7543(88)90024-9.
The gene for cystic fibrosis (CF) is known to be flanked by the closely linked DNA markers met and J3.11 on chromosome 7. Using the technique of pulsed-field gel electrophoresis, we have constructed a complete overlapping restriction map of approximately 3000 kb of DNA in this region. The met and J3.11 probes are found to be between 1300 and 1800 kb apart, which compares well with their genetic distance of 1-2 cM. The CF gene must be located within this interval, and the availability of this physical map should be of considerable utility in mapping additional clones as the search for the gene proceeds.
已知囊性纤维化（CF）基因位于7号染色体上紧密连锁的DNA标记met和J3.11之间。利用脉冲场凝胶电泳技术，我们构建了该区域约3000 kb DNA的完整重叠限制酶切图谱。发现met和J3.11探针相距1300至1800 kb，这与其1 - 2 cM的遗传距离相当吻合。CF基因必定位于这个区间内，随着寻找该基因工作的推进，这一物理图谱的可得性对于定位其他克隆将具有相当大的实用价值。
