Safdari Faezeh, Rabbani Mohammad, Hosseini-Sharifabad Ali
Department of Pharmacology and Toxicology and Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, I.R. Iran.
Res Pharm Sci. 2017 Apr;12(2):154-159. doi: 10.4103/1735-5362.202454.
Potassium bromide (KBr), an old antiepileptic agent, is illegally used in pharmaceutical or food industries to improve the product appearance. KBr has been proven to influence several pathways which are important in memory formation. Therefore, the present study aimed to evaluate the effect of KBr on spatial working memory using object recognition task (ORT). Rats received a single dose of KBr (50, 100 or 150 mg/kg), per oral, in acute treatment. KBr long term effects were also studied in animals receiving 50 mg/kg/day of KBr for 28 consecutive days. At the end of treatments, animals underwent two trials of ORT, five min each. In the first trial (T), animals encountered with two identical objects for exploration. After 1 h, the animals were exposed to a familiar and an unfamiliar object (T). The exploration times for discrimination (D) and recognition (R) as well as the frequency of exploration for any objects were determined. Acute administration of 150 mg/kg of KBr significantly decreased the discrimination and recognition indices (RI and DI) ( < 0.01) compared to the control. However, lower doses failed to influence the animals' performance in the test. In addition, long term administration of KBr remarkably diminished the DI and RI and the frequency of exploration ( < 0.05). The results of this study indicate that acute doses of KBr as high as 150 mg/kg are required to hamper memory function in ORT. However, cognitive impairment occured with lower doses of KBr when the duration of treatment is extended.
溴化钾（KBr）是一种古老的抗癫痫药物，被非法用于制药或食品工业以改善产品外观。已证实KBr会影响记忆形成中重要的几种途径。因此，本研究旨在使用物体识别任务（ORT）评估KBr对空间工作记忆的影响。大鼠在急性治疗中口服单剂量KBr（50、100或150mg/kg）。还对连续28天接受50mg/kg/天KBr的动物进行了KBr长期影响的研究。在治疗结束时，动物进行了两次ORT试验，每次5分钟。在第一次试验（T）中，动物遇到两个相同的物体进行探索。1小时后，动物接触一个熟悉的物体和一个不熟悉的物体（T）。确定了辨别（D）和识别（R）的探索时间以及对任何物体的探索频率。与对照组相比，急性给予150mg/kg的KBr显著降低了辨别和识别指数（RI和DI）（<0.01）。然而，较低剂量未能影响动物在测试中的表现。此外，长期给予KBr显著降低了DI和RI以及探索频率（<0.05）。本研究结果表明，在ORT中需要高达150mg/kg的急性剂量KBr才能损害记忆功能。然而，当治疗时间延长时，较低剂量的KBr会导致认知障碍。
