Batool Zehra, Sadir Sadia, Liaquat Laraib, Tabassum Saiqa, Madiha Syeda, Rafiq Sahar, Tariq Sumayya, Batool Tuba Sharf, Saleem Sadia, Naqvi Fizza, Perveen Tahira, Haider Saida
Neurochemistry and Biochemical Neuropharmacology Research Unit, Department of Biochemistry, University of Karachi, Karachi 75270, Pakistan.
Department of Agriculture and Agribusiness Management, University of Karachi, Karachi 75270, Pakistan.
Brain Res Bull. 2016 Jan;120:63-74. doi: 10.1016/j.brainresbull.2015.11.001. Epub 2015 Nov 5.
Dietary nutrients may play a vital role in protecting the brain from age-related memory dysfunction and neurodegenerative diseases. Tree nuts including almonds have shown potential to combat age-associated brain dysfunction. These nuts are an important source of essential nutrients, such as tocopherol, folate, mono- and poly-unsaturated fatty acids, and polyphenols. These components have shown promise as possible dietary supplements to prevent or delay the onset of age-associated cognitive dysfunction. This study investigated possible protective potential of almond against scopolamine induced amnesia in rats. The present study also investigated a role of acetylcholine in almond induced memory enhancement. Rats in test group were orally administrated with almond suspension (400 mg/kg/day) for four weeks. Both control and almond-treated rats were then divided into saline and scopolamine injected groups. Rats in the scopolamine group were injected with scopolamine (0.5 mg/kg) five minutes before the start of each memory test. Memory was assessed by elevated plus maze (EPM), Morris water maze (MWM) and novel object recognition (NOR) task. Cholinergic function was determined in terms of hippocampal and frontal cortical acetylcholine content and acetylcholinesterase activity. Results of the present study suggest that almond administration for 28 days significantly improved memory retention. This memory enhancing effect of almond was also observed in scopolamine induced amnesia model. Present study also suggests a role of acetylcholine in the attenuation of scopolamine induced amnesia by almond.
膳食营养成分在保护大脑免受与年龄相关的记忆功能障碍和神经退行性疾病方面可能起着至关重要的作用。包括杏仁在内的坚果已显示出对抗与年龄相关的大脑功能障碍的潜力。这些坚果是必需营养素的重要来源，如生育酚、叶酸、单不饱和脂肪酸和多不饱和脂肪酸以及多酚。这些成分有望作为可能的膳食补充剂来预防或延缓与年龄相关的认知功能障碍的发生。本研究调查了杏仁对东莨菪碱诱导的大鼠失忆的可能保护潜力。本研究还调查了乙酰胆碱在杏仁诱导的记忆增强中的作用。试验组大鼠口服杏仁混悬液（400毫克/千克/天），持续四周。然后将对照组和杏仁处理组大鼠均分为生理盐水注射组和东莨菪碱注射组。东莨菪碱组大鼠在每次记忆测试开始前五分钟注射东莨菪碱（0.5毫克/千克）。通过高架十字迷宫（EPM）、莫里斯水迷宫（MWM）和新物体识别（NOR）任务评估记忆。根据海马和额叶皮质乙酰胆碱含量以及乙酰胆碱酯酶活性来确定胆碱能功能。本研究结果表明，给予杏仁28天可显著改善记忆保持。在东莨菪碱诱导的失忆模型中也观察到了杏仁的这种记忆增强作用。本研究还表明乙酰胆碱在杏仁减轻东莨菪碱诱导的失忆中发挥了作用。
