Zachut M, Kra G, Livshitz L, Portnick Y, Yakoby S, Friedlander G, Levin Y
Department of Ruminant Science, Institute of Animal Sciences, Volcani Center, Rishon Lezion, Israel.
The Ilana and Pascal Mantoux Institute for Bioinformatics, The Nancy and Stephen Grand Israel National Center for Personalized Medicine, Weizmann Institute of Science, Rehovot, Israel.
Data Brief. 2017 May 4;12:535-539. doi: 10.1016/j.dib.2017.04.042. eCollection 2017 Jun.
Adipose tissue has a central role in the regulation of metabolism in dairy cows, and many proteins expressed in this tissue are involved in metabolic responses to stress (Peinado et al., 2012) [1]. Environmental heat stress is one of the main stressors limiting production in dairy cattle (Fuquay, 1981; West, 2003) [2], [3], and there is a complex interaction between heat stress and the transition period from late pregnancy to onset of lactation, which is manifested in heat-stressed late-gestation cows (Tao and Dahl, 2013) [4]. We recently defined the proteome of adipose tissue in peripartum dairy cows, identifying 586 proteins of which 18.9% were differentially abundant in insulin-resistant compared to insulin-sensitive adipose tissue (Zachut, 2015) [5]. That study showed that proteomic techniques constitute a valuable tool for identifying novel biomarkers in adipose tissue that are related to metabolic adaptation to stress in dairy cows. The objective of the present work was to examine the adipose tissue proteome under thermo-neutral or seasonal heat stress conditions in late pregnant dairy cows. We have collected subcutaneous adipose tissue biopsies from 10 late pregnant dairy cows during summer heat stress and from 8 late pregnant dairy cows during winter season, and identified and quantified 1495 proteins in the adipose tissues. This dataset of adipose tissue proteome from dairy cows adds novel information on the variety of proteins that are abundant in this tissue during late pregnancy under thermo-neutral as well as heat stress conditions. Differential abundance of 107 (7.1%) proteins was found between summer and winter adipose. These results are discussed in our recent research article (Zachut et al., 2017) [6].
脂肪组织在奶牛新陈代谢的调节中起着核心作用，该组织中表达的许多蛋白质都参与了对应激的代谢反应（Peinado等人，2012年）[1]。环境热应激是限制奶牛生产的主要应激源之一（Fuquay，1981年；West，2003年）[2]，[3]，热应激与从妊娠后期到泌乳开始的过渡期之间存在复杂的相互作用，这在热应激的妊娠后期奶牛中表现出来（Tao和Dahl，2013年）[4]。我们最近确定了围产期奶牛脂肪组织的蛋白质组，鉴定出586种蛋白质，其中18.9%在胰岛素抵抗型脂肪组织中与胰岛素敏感型脂肪组织相比丰度存在差异（Zachut，2015年）[5]。该研究表明，蛋白质组学技术是识别脂肪组织中与奶牛代谢适应应激相关的新型生物标志物的宝贵工具。本研究的目的是检查妊娠后期奶牛在热中性或季节性热应激条件下的脂肪组织蛋白质组。我们在夏季热应激期间从10头妊娠后期奶牛和冬季从8头妊娠后期奶牛采集了皮下脂肪组织活检样本，并鉴定和定量了脂肪组织中的1495种蛋白质。这个奶牛脂肪组织蛋白质组数据集增加了关于在热中性以及热应激条件下妊娠后期该组织中丰富的各种蛋白质的新信息。在夏季和冬季脂肪组织之间发现了107种（7.1%）蛋白质的丰度差异。这些结果在我们最近的研究文章中进行了讨论（Zachut等人，2017年）[6]。
