Reetz I C, Wullenweber-Schmidt M, Kraft V, Hedrich H J
Section of Genetics and Reproduction Biology, Central Institute for Laboratory Animal Breeding, Hannover, F.R.G.
Lab Anim Sci. 1988 Dec;38(6):696-701.
Embryo transfers were performed to rederive six inbred strains of mice, A/He, BALB/cByJ, BALB/c Lac, B10.BR/SgSnJ, C57BL/6J and DBA/2J. The aim was to determine whether it is possible to eliminate pathogens like mouse hepatitis virus (MHV) and Pasteurella pneumotropica (P.p.). The embryos were collected, handled and transferred into the oviduct of day one pseudopregnant SPF surrogate mothers under aseptic conditions. In 40.5% of the transfers, embryos developed to term. With respect to surrogate mothers delivering viable litters, 47.9% of the transferred embryos were born alive. Out of these 93.5% were reared. Virological and bacteriological examination of embryo donors verified the presence of P.p. and of antibodies against MHV in all strains. In some embryo donors P.p. could be isolated even from the uterine mucosa. However, neither in the surrogate mothers nor in the offspring could P.p. and antibodies against MHV be detected. Further bacteriological examination revealed that the offspring carried only the microbial flora received from the surrogate mother. The results indicate that embryo transfer is an appropriate tool to rederive mouse strains. In contrast to hysterectomy rederivation, embryo transfer has the advantage of avoiding postimplantational vertical transmissions of infections.
进行胚胎移植以重新培育6个近交系小鼠，即A/He、BALB/cByJ、BALB/c Lac、B10.BR/SgSnJ、C57BL/6J和DBA/2J。目的是确定是否有可能消除诸如小鼠肝炎病毒（MHV）和嗜肺巴斯德杆菌（P.p.）等病原体。胚胎在无菌条件下采集、处理并移植到第1天假孕的无特定病原体（SPF）代孕母鼠的输卵管中。在40.5%的移植中，胚胎发育至足月。关于代孕母鼠产下存活的一窝小鼠，47.9%的移植胚胎存活出生。其中93.5%得以饲养。对胚胎供体的病毒学和细菌学检查证实所有品系中均存在P.p.和抗MHV抗体。在一些胚胎供体中，甚至可从子宫黏膜分离出P.p.。然而，无论是在代孕母鼠还是后代中，均未检测到P.p.和抗MHV抗体。进一步的细菌学检查显示，后代仅携带从代孕母鼠获得的微生物菌群。结果表明，胚胎移植是重新培育小鼠品系的一种合适工具。与子宫切除重新培育相比，胚胎移植具有避免植入后感染垂直传播的优势。
