Julius Kühn-Institute (JKI), Federal Research Centre for Cultivated Plants, Institute for Resistance Research and Stress Tolerance, Erwin-Baur-Str. 27, 06484, Quedlinburg, Germany.
Plant Breeding Department, IFZ Research Centre for Biosystems, Land Use and Nutrition, Justus Liebig University, Heinrich-Buff-Ring 26-32, 35392, Gießen, Germany.
New Phytol. 2017 Jul;215(2):779-791. doi: 10.1111/nph.14595. Epub 2017 May 18.
Bread wheat (Triticum aestivum) is a major staple food and therefore of prime importance for feeding the Earth's growing population. Mycorrhiza is known to improve plant growth, but although extensive knowledge concerning the interaction between mycorrhizal fungi and plants is available, genotypic differences concerning the ability of wheat to form mycorrhizal symbiosis and quantitative trait loci (QTLs) involved in mycorrhization are largely unknown. Therefore, a diverse set of 94 bread wheat genotypes was evaluated with regard to root colonization by arbuscular mycorrhizal fungi. In order to identify genomic regions involved in mycorrhization, these genotypes were analyzed using the wheat 90k iSelect chip, resulting in 17 823 polymorphic mapped markers, which were used in a genome-wide association study. Significant genotypic differences (P < 0.0001) were detected in the ability to form symbiosis and 30 significant markers associated with root colonization, representing six QTL regions, were detected on chromosomes 3A, 4A and 7A, and candidate genes located in these QTL regions were proposed. The results reported here provide key insights into the genetics of root colonization by mycorrhizal fungi in wheat.
面包小麦（Triticum aestivum）是主要的主食，因此对养活不断增长的世界人口至关重要。根瘤菌已知能促进植物生长，但尽管人们对根瘤菌真菌与植物之间的相互作用有广泛的了解，但关于小麦形成根瘤菌共生的基因型差异和参与根瘤菌形成的数量性状位点（QTL）在很大程度上仍不清楚。因此，评估了 94 种不同的面包小麦基因型的根被丛枝菌根真菌定殖的情况。为了鉴定与菌根化相关的基因组区域，使用小麦 90k iSelect 芯片对这些基因型进行了分析，得到了 17823 个多态性标记，并在全基因组关联研究中进行了分析。在形成共生的能力方面，检测到显著的基因型差异（P<0.0001），并在染色体 3A、4A 和 7A 上检测到与根定殖相关的 30 个显著标记，代表 6 个 QTL 区域，并提出了位于这些 QTL 区域的候选基因。这里报道的结果为小麦中丛枝菌根真菌定殖的遗传学提供了重要的见解。
