Department of Crop Science, University of Illinois, 1101 W. Peabody Drive, Urbana, IL, 61801, USA.
Department of Plant Sciences, University of Missouri, Columbia, MO, USA.
Theor Appl Genet. 2020 Feb;133(2):409-417. doi: 10.1007/s00122-019-03471-5. Epub 2019 Nov 9.
A whole-genome resequencing-derived SNP dataset identified six quantitative trait loci (QTL) significantly associated with colonization of soybean by an arbuscular mycorrhizal fungus (Rhizophagus intraradices). Candidate genes identified in these QTL regions include homologs to known nodulin protein families and other symbiosis-specific genes. Arbuscular mycorrhizal fungi (AMF) form associations with over 80% of all terrestrial plant species and assist their host plants by increasing their nutrient uptake, drought tolerance, and resilience against pathogens and pests. Genotypic variation of crop plants to AMF colonization has been identified in crops, including soybean; however, the genetics controlling levels of AMF colonization in soybean are unknown. The overall goal of our study was to identify genomic regions associated with mycorrhizal colonization in soybean using genome-wide association analysis. A diverse panel of 350 exotic soybean genotypes inoculated with Rhizophagus intraradices were microscopically evaluated for root colonization using a modified gridline intersect method. Root colonization differed significantly (P < 0.001) among genotypes and ranged from 11 to 70%. A whole-genome resequencing-derived SNP dataset identified six quantitative trait loci (QTL) significantly associated with R. intraradices colonization that explained 24% of the phenotypic variance. Candidate genes identified in these QTL regions include homologs to known nodulin protein families and other symbiosis-specific genes. The results showed there was a significant genetic component to the level of colonization by R. intraradices in soybean. This information may be useful in the development of AMF-sensitive soybean cultivars to enhance nutrient uptake, drought tolerance, and disease resistance in the crop.
全基因组重测序衍生 SNP 数据集确定了 6 个与丛枝菌根真菌（Rhizophagus intraradices）定殖大豆显著相关的数量性状位点（QTL）。在这些 QTL 区域中鉴定出的候选基因包括已知豆球蛋白蛋白家族和其他共生特异性基因的同源物。丛枝菌根真菌（AMF）与超过 80%的所有陆地植物物种形成共生关系，并通过增加宿主植物的养分吸收、耐旱性以及对病原体和害虫的抵抗力来帮助它们。在包括大豆在内的作物中已经确定了作物对 AMF 定殖的基因型变异；然而，控制大豆中 AMF 定殖水平的遗传机制尚不清楚。我们研究的总体目标是使用全基因组关联分析来鉴定与大豆共生定殖相关的基因组区域。使用改良的网格线交叉法，对接种 Rhizophagus intraradices 的 350 种异源大豆基因型进行了根定殖的显微镜评估。根定殖在基因型之间存在显著差异（P<0.001），范围从 11%到 70%不等。全基因组重测序衍生 SNP 数据集确定了 6 个与 R. intraradices 定殖显著相关的数量性状位点（QTL），这些 QTL 解释了 24%的表型方差。在这些 QTL 区域中鉴定出的候选基因包括已知豆球蛋白蛋白家族和其他共生特异性基因的同源物。结果表明，大豆中 R. intraradices 的定殖水平存在显著的遗传成分。这些信息可能有助于开发对 AMF 敏感的大豆品种，以增强作物的养分吸收、耐旱性和抗病性。
