Primary structure of the regulatory gene from the MAL6 locus of Saccharomyces carlsbergensis.

We determined the complete nucleotide sequence of the yeast MAL6R gene from the Saccharomyces carlsbergensis MAL6 locus. The MAL6R gene encodes a transacting protein required for the inducible, coordinate expression of the two divergently transcribed structural genes, MAL6T (maltose permease), and MAL6S (maltase) at this locus. The transcription initiation sites for MAL6R were determined by primer extension experiments. The MAL6R gene contains an open reading frame of 473 amino acids with a calculated Mr of 54,892. The N-terminus of the deduced protein contains an amino acid sequence isologous to a consensus sequence for cysteine-zinc associated DNA binding fingers found in other fungal DNA binding proteins. The MAL6R gene was mapped to chromosome VIII by using OFAGE (orthagonal field alternating gel electrophoresis) gels and hybridization with specific chromosome and MAL6 probes.