Jiang Liu, Qian Huaming, Chen Gang, Li Chunyan, Yan Guangjun, Luo Yanling, Liu Peng, Chen Yashao
1 Key Laboratory of Applied Surface and Colloid Chemistry of Ministry of Education, School of Chemistry & Chemical Engineering, Shaanxi Normal University, Xi'an, China.
2 School of Foreign Languages, Shaanxi Normal University, Xi'an, China.
J Biomater Appl. 2017 May;31(10):1346-1357. doi: 10.1177/0885328217707100.
In this study, micropatterns on polypropylene films were fabricated via plasma pretreatment and UV-initiated graft polymerization. Firstly, radio-frequency plasma, which does not significantly influence bulk attributes of substrates due to limited penetration depth, was utilized to activate polypropylene films. Then, different sizes of micropatterns of poly(hydroxyethyl methacrylate) (PHEMA) were fabricated on the polypropylene films via UV-initiated graft polymerization of hydroxyethyl methacrylate by using photo-masks. Scanning electron microscopy, atomic force microscopy, X-ray photoelectron spectroscopy, and contact angle (CA) were employed to characterize changes of pristine polypropylene films and modified ones in surface morphology, roughness, hydrophilicity, free energy and the surface chemical composition. All of these confirmed the successful grafting of different sizes of PHEMA micropatterns on the polypropylene surface. Furthermore, the influence of PHEMA micropatterns on cell proliferation and cytotoxicity was evaluated in vitro. Analysis of cell behaviour indicated that PHEMA micropatterns of the appropriate size can promote cellular adhesion and proliferation, and the PHEMA-micropatterned polypropylene films had good biocompatibility. The approach presented here provides an alternative to synthesize on the surface of polypropylene films' micropatterns with the aim of using them in a diverse array of applications.
在本研究中，通过等离子体预处理和紫外光引发接枝聚合在聚丙烯薄膜上制备了微图案。首先，利用射频等离子体激活聚丙烯薄膜，由于其穿透深度有限，不会显著影响基底的整体属性。然后，通过使用光掩模，通过甲基丙烯酸羟乙酯的紫外光引发接枝聚合，在聚丙烯薄膜上制备了不同尺寸的聚（甲基丙烯酸羟乙酯）（PHEMA）微图案。采用扫描电子显微镜、原子力显微镜、X射线光电子能谱和接触角（CA）来表征原始聚丙烯薄膜和改性薄膜在表面形貌、粗糙度、亲水性、自由能和表面化学成分方面的变化。所有这些都证实了不同尺寸的PHEMA微图案成功接枝到聚丙烯表面。此外，还在体外评估了PHEMA微图案对细胞增殖和细胞毒性的影响。细胞行为分析表明，适当尺寸的PHEMA微图案可以促进细胞粘附和增殖，且PHEMA微图案化的聚丙烯薄膜具有良好的生物相容性。本文提出的方法为在聚丙烯薄膜表面合成微图案提供了一种替代方案，旨在将其用于各种应用中。
