Żukowski Maciej, Taryma-Leśniak Olga, Kaczmarczyk Mariusz, Kotfis Katarzyna, Szydłowski Łukasz, Ciechanowicz Andrzej, Brykczyński Mirosław, Żukowska Agnieszka
Department of Anaesthesiology, Intensive Therapy and Acute Intoxications Pomeranian Medical University in Szczecin.
Anaesthesiol Intensive Ther. 2017;49(2):110-115. doi: 10.5603/AIT.a2017.0027. Epub 2017 May 18.
The association among specific single-nucleotide polymorphisms (SNPs) in TLR2 R753Q (rs5743708) and T16934A (rs4696480) and the nasal carriage of Staphylococcus aureus was studied in adults before CABG.
The TLR2 polymorphisms were genotyped in 299 consecutive patients prepared for a CABG operation. Genotyping was performed using restriction fragment length polymorphism (RFLP) analysis of PCR-amplified fragments. Two nasal swab cultures were taken within 2 weeks before the operation. Subjects were classified as Staphylococcus aureus carriers if at least one culture was positive while those patients with both cultures found to be negative were classified as non-carriers.
The prevalence of nasal S. aureus carriage in the final cohort was 22.1% (66/299), while no MRSA was detected in our study group. No significant differences in the TLR2 polymorphisms were observed between the study and the control groups. No associations were found between TLR2 haplotypes and the covariates of age, sex, NYHA, weight, height, BMI, CAD, smoking status and ESlog score. No differences were found between carriers and noncarriers regarding the allelic distribution of the TLR2 T-16934A SNP. Almost 93% of the patients who were screened for the presence of the TLR2 Arg753Gln (rs5743708) were GG wild type homozygous. Twenty one subjects from the study group (7.1%) were GA heterozygous, while no patient in either group was homozygous for the TLR2 Arg753Gln (rs5743708) mutation. TLR2 Arg753Gln genotyping showed that GA heterozygous patients were detected more frequently in the group of Staphylococcus aureus nasal carriers than in non-carrier adults.
Our results suggest that the carrier status for the GA variant of the TLR2 Arg753Gln (rs5743708) polymorphism may be a risk factor for Staphylococcus aureus carriage.
在成人冠状动脉旁路移植术（CABG）前，研究了TLR2基因R753Q（rs5743708）和T16934A（rs4696480）中特定单核苷酸多态性（SNP）与金黄色葡萄球菌鼻腔携带之间的关联。
对299例连续准备接受CABG手术的患者进行TLR2基因多态性基因分型。采用聚合酶链反应（PCR）扩增片段的限制性片段长度多态性（RFLP）分析进行基因分型。在手术前2周内采集两份鼻拭子培养物。如果至少一份培养物呈阳性，则受试者被分类为金黄色葡萄球菌携带者；而两份培养物均为阴性的患者则被分类为非携带者。
最终队列中鼻腔金黄色葡萄球菌携带率为22.1%（66/299），而在我们的研究组中未检测到耐甲氧西林金黄色葡萄球菌（MRSA）。研究组和对照组之间在TLR2基因多态性方面未观察到显著差异。在TLR2单倍型与年龄、性别、纽约心脏协会（NYHA）心功能分级、体重、身高、体重指数（BMI）、冠状动脉疾病（CAD）、吸烟状况和欧洲心脏手术风险评估系统（ESlog）评分等协变量之间未发现关联。在TLR2 T-16934A SNP的等位基因分布方面，携带者和非携带者之间未发现差异。在接受TLR2 Arg753Gln（rs5743708）检测的患者中，近93%为GG野生型纯合子。研究组中有21名受试者（7.1%）为GA杂合子，而两组中均无患者为TLR2 Arg753Gln（rs5743708）突变的纯合子。TLR2 Arg753Gln基因分型显示，金黄色葡萄球菌鼻腔携带者组中GA杂合子患者的检出频率高于非携带者成人组。
我们的结果表明，TLR2 Arg753Gln（rs5743708）多态性的GA变异携带者状态可能是金黄色葡萄球菌携带的一个危险因素。
