Department of Otorhinolaryngology, Head and Neck Surgery, University of Münster, D-48149 Muenster, Germany.
Am J Rhinol Allergy. 2010 Jan-Feb;24(1):e10-3. doi: 10.2500/ajra.2010.24.3416.
The impact of Staphylococcus aureus on the development of chronic sinusitis with nasal polyps (nasal polyposis [NP]) is a controversial discussion because different S. aureus colonization rates have been reported. Aside from the presence of a microbial stimulus, elements of innate immunity such as Toll-like receptors (TLRs) and/or impaired TLR function could be relevant for the development of this disease. Because the 753Q TLR2 variant may predispose to staphylococcal infection, we simultaneously analyzed staphylococcal colonization and the R753Q TLR2 single nucleotide polymorphism (SNP) in NP.
Sixty-eight patients with NP (47 men and 21 women; mean age [+/-SD], 51.8 years [16.3]) and 51 controls (32 men and 19 women; mean age [+/-SD], 36.3 years [12.2]) were included. Patient characteristics studied included status of allergy, asthma, aspirin intolerance, and endoscopic and CT polyp score. For detection of bacteria, standard procedures of bacteriology and 16S rRNA gene sequencing were used. The R753Q TLR2 polymorphism was studied by allelic discrimination assay.
Overall, 128 isolates were cultured from 68 NP specimens, with Staphylococcus epidermidis and S. aureus being the most frequent bacterial isolates. Other bacterial species were infrequently detected. Fifty-nine isolates were cultured from 51 controls. Similarly, S. epidermidis and S. aureus were the most frequent bacterial isolates. S. aureus colonization was significantly increased in NP (p < 0.05). However, SNP genotyping results showed no association of the 753Q TLR2 variant with NP.
Although S. aureus detection was increased in NP, nasal polyp pathology is not related to the 753Q TLR2 variant.
金黄色葡萄球菌对慢性鼻鼻窦炎伴鼻息肉（鼻息肉[NP]）发展的影响是一个有争议的讨论，因为报道的金黄色葡萄球菌定植率不同。除了微生物刺激物的存在之外，先天免疫的元素，如 Toll 样受体（TLR）和/或 TLR 功能受损，可能与这种疾病的发展有关。由于 753Q TLR2 变体可能易感染金黄色葡萄球菌，我们同时分析了 NP 中的金黄色葡萄球菌定植和 R753Q TLR2 单核苷酸多态性（SNP）。
纳入 68 例 NP 患者（47 名男性和 21 名女性；平均年龄[+/-SD]，51.8 岁[16.3]）和 51 名对照者（32 名男性和 19 名女性；平均年龄[+/-SD]，36.3 岁[12.2]）。研究的患者特征包括过敏、哮喘、阿司匹林不耐受以及内镜和 CT 息肉评分的状态。为了检测细菌，采用了细菌学的标准程序和 16S rRNA 基因测序。通过等位基因鉴别检测研究 R753Q TLR2 多态性。
总体而言，从 68 例 NP 标本中培养了 128 株分离物，表皮葡萄球菌和金黄色葡萄球菌是最常见的细菌分离物。其他细菌种属很少被检测到。从 51 名对照中培养了 59 株分离物。同样，表皮葡萄球菌和金黄色葡萄球菌是最常见的细菌分离物。NP 中金黄色葡萄球菌定植显著增加（p < 0.05）。然而，SNP 基因分型结果显示 753Q TLR2 变体与 NP 无关。
尽管 NP 中金黄色葡萄球菌的检测增加，但鼻息肉病理与 753Q TLR2 变体无关。
