Tischer I, Buhk H J
Robert Koch-Institut, Bundesgesundheitamt, Berlin.
Zentralbl Bakteriol Mikrobiol Hyg A. 1988 Nov;270(1-2):280-7. doi: 10.1016/s0176-6724(88)80164-0.
It has been demonstrated earlier that the genome of the porcine circovirus (PCV) isolated from virions is a circular single-stranded DNA (ssDNA). This report describes the isolation and characterization of PCV-specific DNA from infected cell cultures. Two types of double-stranded DNA (dsDNA) were observed which behaved like supercoiled and relaxed circular molecules, respectively. Both types of dsDNA displayed infectivity in transfection experiments and are regarded as replicative forms (RF) of the viral genome. In addition to complete single-stranded viral genomes and RF DNA molecules, PCV-specific subgenomic fractions of DNA molecules with a sedimentation coefficient of about 5 S were isolated from infected tissue culture cells. This 5 S DNA was also isolated from purified virions. It is single-stranded and represents a defined region of the viral genome.
先前已证明，从病毒粒子中分离出的猪圆环病毒（PCV）基因组是环状单链DNA（ssDNA）。本报告描述了从感染的细胞培养物中分离和鉴定PCV特异性DNA的过程。观察到两种双链DNA（dsDNA），它们分别表现为超螺旋和松弛环状分子。两种类型的dsDNA在转染实验中均显示出感染性，被视为病毒基因组的复制形式（RF）。除了完整的单链病毒基因组和RF DNA分子外，还从感染的组织培养细胞中分离出沉降系数约为5 S的PCV特异性亚基因组DNA片段。这种5 S DNA也从纯化的病毒粒子中分离出来。它是单链的，代表病毒基因组的一个特定区域。
