Pradal G, Lefranc G, Piloquet P, Vo N H, Riet A
Laboratoire d'Histologie, Faculté de Médecine, Nantes, France.
Biol Cell. 1988;64(1):71-7. doi: 10.1016/0248-4900(88)90095-0.
In endocrine (EC) cells of rabbit fundic mucosa, it is practically impossible to obtain unequivocal ultrastructural identification of all cells found in order to perform morphometric analysis. In addition to classic EC cells with pleomorphic granules, a cell type with entirely round granules is encountered which can be confused with non-EC cells. To solve this problem, all EC cells in our study were first identified by their 5-HT (immunocytochemistry) and argentaffinity. Examination of the fine structures of reactive cells then revealed that the round granules of EC cells were differentiated from those of non-EC cells by the existence of a dense core surrounded by a less dense halo, a feature providing unequivocal ultrastructural identification. EC cells with round granules showed less argentaffinity and less immunoreactivity to 5-HT as compared with classic EC cells. After labelling with [3H]L-dopa, EC cells with round-granules displayed an overall staining index higher than that of classic EC cells and comparable with that of D cells; however, the nuclear staining index was higher than that of D cells.
在兔胃底黏膜的内分泌（EC）细胞中，实际上不可能对所有发现的细胞进行明确的超微结构鉴定以进行形态计量分析。除了具有多形性颗粒的经典EC细胞外，还会遇到一种具有完全圆形颗粒的细胞类型，它可能会与非EC细胞混淆。为了解决这个问题，我们研究中的所有EC细胞首先通过其5-羟色胺（免疫细胞化学）和嗜银性进行鉴定。然后对反应性细胞的精细结构进行检查发现，EC细胞的圆形颗粒与非EC细胞的圆形颗粒的区别在于存在一个由较不致密的晕圈包围的致密核心，这一特征提供了明确的超微结构鉴定依据。与经典EC细胞相比，具有圆形颗粒的EC细胞显示出较低的嗜银性和对5-羟色胺的较低免疫反应性。用[3H]左旋多巴标记后，具有圆形颗粒的EC细胞的总体染色指数高于经典EC细胞，与D细胞相当；然而，核染色指数高于D细胞。
