Zaviacíc M, Brozman M, Jakubovský J
Histochemistry. 1976 Nov 12;49(4):315-25. doi: 10.1007/BF00496135.
The ultrastructure and certain cytochemical parameters of endocrine cells of the rat gastric mucosa during 168 h of fasting were investigated. To some of the fasting animals peroral food or alcohol was administered before decapitation. The EC (enterochromaffin cells) the ECL (enterochromaffin-like cells), D1 cells, AL (A-like cells) and G cells were identified by means of electron microscopy. Only the EC, ECL, and G cells could be identified by means of light microscopy by an adequate histochemical technique. The ultrastructural picture of the ECL and of the EC cells did not change markedly during the fasting. In the D1 cells there occurred an agglomeration of secretory granules. Some of them disintegrated and disappeared. In the AL cells an agglomeration of granules during the fasting was also observed. Granules engulfed in lysosomes were often found. The participation of lysosomes in the degradation of granules during the fasting was more marked in the AL cells than in the G cells. The participation of lysosomes was questionable in the EC and D1 cells, and in the ECL cells no lysosomes were observed. In contradistinction to the G cells of the non-fasting animals, where more than one half of the gastrin granules were "empty", the G cells during the fasting were filled with agglomerated dense granules and contained lysosomes with fragments of engulfed secretory granules. Following the administration of food (Larsen's diet) 3 h before sacrificing the dissolution of the content of granules with well preserved membranes was observed (emiocytosis did not take place). The administration of food did not lead to changes in the ultrastructural appearance of the EC cells. The peroral administration of alcohol did not lead to any changes in the ultrastructural appearance of the AL and G cells.
研究了大鼠胃黏膜内分泌细胞在禁食168小时期间的超微结构和某些细胞化学参数。在断头前，给部分禁食动物经口给予食物或酒精。通过电子显微镜鉴定了肠嗜铬细胞（EC细胞）、肠嗜铬样细胞（ECL细胞）、D1细胞、A样细胞（AL细胞）和G细胞。只有通过适当的组织化学技术，才能利用光学显微镜鉴定出EC细胞、ECL细胞和G细胞。在禁食期间，ECL细胞和EC细胞的超微结构图像没有明显变化。D1细胞中出现了分泌颗粒的聚集。其中一些颗粒解体并消失。在禁食期间，AL细胞中也观察到颗粒的聚集。经常发现溶酶体吞噬颗粒。禁食期间，溶酶体在AL细胞中对颗粒降解的参与比在G细胞中更明显。溶酶体在EC细胞和D1细胞中的参与情况存在疑问，在ECL细胞中未观察到溶酶体。与非禁食动物的G细胞不同，非禁食动物中超过一半的胃泌素颗粒是“空的”，而禁食期间的G细胞充满了聚集的致密颗粒，并含有带有被吞噬分泌颗粒片段的溶酶体。在处死前3小时给予食物（拉森饮食）后，观察到颗粒内容物溶解且膜保存完好（未发生胞吐作用）。给予食物并未导致EC细胞超微结构外观的改变。经口给予酒精并未导致AL细胞和G细胞超微结构外观的任何改变。
