Matsumura Yasufumi, Peirano Gisele, Devinney Rebekah, Bradford Patricia A, Motyl Mary R, Adams Mark D, Chen Liang, Kreiswirth Barry, Pitout Johann D D
Department of Microbiology, Immunology and Infectious Diseases, University of Calgary, Calgary, Alberta, Canada.
Department of Clinical Laboratory Medicine, Kyoto University Graduate School of Medicine, Kyoto, Japan.
J Antimicrob Chemother. 2017 Aug 1;72(8):2249-2258. doi: 10.1093/jac/dkx148.
International data on the molecular epidemiology of Enterobacteriaceae with VIM carbapenemases are limited.
We performed short read (Illumina) WGS on a global collection of 89 VIM-producing clinical Enterobacteriaceae (2008-14).
VIM-producing (11 varieties within 21 different integrons) isolates were mostly obtained from Europe. Certain integrons with bla VIM were specific to a country in different species and clonal complexes (CCs) (In 87 , In 624 , In 916 and In 1323 ), while others had spread globally among various Enterobacteriaceae species (In 110 and In 1209 ). Klebsiella pneumoniae was the most common species ( n = 45); CC147 from Greece was the most prevalent clone and contained In 590 -like integrons with four different bla VIM s. Enterobacter cloacae complex was the second most common species and mainly consisted of Enterobacter hormaechei ( Enterobacter xiangfangensis , subsp. steigerwaltii and Hoffmann cluster III). CC200 (from Croatia and Turkey), CC114 (Croatia, Greece, Italy and the USA) and CC78 (from Greece, Italy and Spain) containing bla VIM-1 were the most common clones among the E. cloacae complex.
This study highlights the importance of surveillance programmes using the latest molecular techniques in providing insight into the characteristics and global distribution of Enterobacteriaceae with bla VIM s.
关于产VIM碳青霉烯酶肠杆菌科细菌分子流行病学的国际数据有限。
我们对2008 - 2014年全球收集的89株产VIM临床分离肠杆菌科细菌进行了短读长（Illumina）全基因组测序（WGS）。
产VIM（21种不同整合子内的11个变种）的分离株大多来自欧洲。某些携带bla VIM的整合子在不同物种和克隆复合体（CCs）中具有国别特异性（In 87、In 624、In 916和In 1323），而其他整合子则在全球各种肠杆菌科细菌中传播（In 110和In 1209）。肺炎克雷伯菌是最常见的物种（n = 45）；来自希腊的CC147是最流行的克隆，包含具有四种不同bla VIM的In 590样整合子。阴沟肠杆菌复合体是第二常见的物种，主要由阪崎肠杆菌（香芳肠杆菌，施氏亚种和霍夫曼III群）组成。包含bla VIM-1的CC200（来自克罗地亚和土耳其）、CC114（克罗地亚、希腊、意大利和美国）和CC78（来自希腊、意大利和西班牙）是阴沟肠杆菌复合体中最常见的克隆。
本研究强调了使用最新分子技术的监测计划对于深入了解携带bla VIM的肠杆菌科细菌特征和全球分布的重要性。
