Wageningen Food & Biobased Research, AlgaePARC, PO Box 17, 6700 AA Wageningen, The Netherlands.
Wageningen Food & Biobased Research, AlgaePARC, PO Box 17, 6700 AA Wageningen, The Netherlands.
Bioresour Technol. 2017 Sep;239:204-210. doi: 10.1016/j.biortech.2017.05.012. Epub 2017 May 5.
Several cell disruption methods were tested on Nannochloropsis gaditana, to evaluate their efficiency in terms of cell disintegration, energy input and release of soluble proteins. High-pressure homogenization (HPH) and bead milling were the most efficient with >95% cell disintegration, ±50% (w/w) release of total proteins and low energy input (<0.5kWh.kg). Enzymatic treatment required low energy input (<0.34kWh.kg), but it only released ±35% protein (w/w). Pulsed Electric Field (PEF) was neither energy-efficient (10.44kWh.kg) nor successful for protein release (only 10% proteins w/w) and cell disintegration. The release of proteins after applying HPH and bead milling always required less intensive operating conditions for cell disruption. The energy cost per unit of released protein ranged from 0.15-0.25 €.kg in case of HPH, and up to 2-20 €.kg in case of PEF.
几种细胞破碎方法在钝顶节旋藻上进行了测试，以评估它们在细胞破碎效率、能量输入和可溶性蛋白质释放方面的效率。高压匀浆（HPH）和珠磨是最有效的方法，细胞破碎率超过 95%，总蛋白质释放率为 ±50%（w/w），能量输入低（<0.5kWh.kg）。酶处理需要的能量输入低（<0.34kWh.kg），但仅释放了 ±35%的蛋白质（w/w）。脉冲电场（PEF）既不节能（10.44kWh.kg），也不能成功释放蛋白质（仅释放 10%的蛋白质 w/w）和细胞破碎。应用 HPH 和珠磨后蛋白质的释放总是需要较低强度的细胞破碎操作条件。每单位释放的蛋白质的能量成本在 HPH 的情况下为 0.15-0.25€.kg，在 PEF 的情况下高达 2-20€.kg。
