Casal C, Infantes J A, Risalde M A, Díez-Guerrier A, Domínguez M, Moreno I, Romero B, de Juan L, Sáez J L, Juste R, Gortázar C, Domínguez L, Bezos J
VISAVET Health Surveillance Centre. Complutense University of Madrid. Avda. Puerta de Hierro s/n, 28040 Madrid, Spain.
VISAVET Health Surveillance Centre. Complutense University of Madrid. Avda. Puerta de Hierro s/n, 28040 Madrid, Spain; Servicio de Inmunología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, 28220 Madrid, Spain.
Res Vet Sci. 2017 Jun;112:214-221. doi: 10.1016/j.rvsc.2017.05.012. Epub 2017 May 11.
We evaluated the sensitivity (Se) of the single cervical intradermal tuberculin (SIT) test, two interferon-gamma (IFN-γ) assays and three different antibody detection techniques for bovine tuberculosis (bTB) diagnosis in 131 mixed beef breed cattle. The results of the diagnostic techniques performed over the whole herd, and over the animals confirmed as infected based on the presence of lesions compatible with the disease and/or M. bovis isolation were compared to determine apparent prevalence (AP) and Se. The Se of the SIT test (severe interpretation) was 63.7% (95% CI, 54.54-72.00), while the Se of the IFN-γ assays ranged between 60.2% and 92%. The proportion of infected cattle detected by the different antibody detection techniques ranged from 65.5% to 87.6%. Three of the antibody detection techniques yielded a significant higher (p<0.05) Se than that achieved with the official diagnostic techniques. In addition, the interpretation in parallel of cellular and antibody detection techniques reached the highest Se: 98.2% (95% CI, 93.78-99.51) suggesting that the use of diagnostic techniques detecting both cellular and humoral responses could be considered as an alternative in the control of bTB outbreaks in high prevalence settings.
我们评估了单剂量颈部皮内结核菌素（SIT）试验、两种干扰素-γ（IFN-γ）检测方法以及三种不同抗体检测技术对131头混种肉牛进行牛结核病（bTB）诊断的敏感性（Se）。将在整个牛群中以及在基于与疾病相符的病变存在和/或牛分枝杆菌分离而确诊感染的动物上所进行的诊断技术结果进行比较，以确定表观患病率（AP）和Se。SIT试验（严格解读）的Se为63.7%（95%置信区间，54.54 - 72.00），而IFN-γ检测方法的Se在60.2%至92%之间。不同抗体检测技术检测出的感染牛比例在65.5%至87.6%之间。三种抗体检测技术的Se显著高于（p<0.05）官方诊断技术。此外，细胞和抗体检测技术并行解读时达到了最高的Se：98.2%（95%置信区间，93.78 - 99.51），这表明在高流行环境中控制bTB疫情时，使用能同时检测细胞和体液反应的诊断技术可被视为一种替代方法。
