Katdare S M, Katdare M, Joshi P N, Mulherkar L
Department of Zoology, University of Poona, Pune, India.
Exp Cell Biol. 1988;56(6):285-8. doi: 10.1159/000163493.
The effect of actinomycin D on chick embryos cultivated in vitro by New's culturing method was studied. Exposure of chick embryos to actinomycin D (0.05 micrograms/ml) at the primitive streak stage (stage 4; Hamburger and Hamilton) for 6 h showed interference in orotic acid formation. The assay of the enzyme dihydroorotic acid dehydrogenase was carried out in both treated and control embryos. No enzymic activity was observed in actinomycin-D-treated embryos in contrast to the considerable activity in the controls. These observations suggest an interference by actinomycin D in the biogenesis of the enzyme dihydroorotic acid dehydrogenase.
研究了放线菌素D对采用纽氏培养法体外培养的鸡胚的影响。在原条期（第4期；汉伯格和汉密尔顿分期）将鸡胚暴露于放线菌素D（0.05微克/毫升）6小时，结果显示乳清酸形成受到干扰。对处理过的和对照胚胎都进行了二氢乳清酸脱氢酶的测定。与对照胚胎中相当可观的酶活性相比，在经放线菌素D处理的胚胎中未观察到酶活性。这些观察结果表明放线菌素D对二氢乳清酸脱氢酶的生物合成有干扰作用。
