Motamed Sadrollah, Taghiabadi Ehsan, Molaei Hojjat, Sodeifi Niloofar, Hassanpour Seyed Esmaeil, Shafieyan Saeed, Azargashb Enzollah, Farajzadeh-Vajari Fatemeh, Aghdami Nasser, Bajouri Amir
Department of Plastic and Reconstructive Surgery, 15th Khordad Hospital, Tehran, Iran.
Skin and Stem Cell Research Center, Tehran University of Medical Sciences, Tehran, Iran; Department of Regenerative Biomedicine, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.
Am J Surg. 2017 Oct;214(4):762-769. doi: 10.1016/j.amjsurg.2017.04.010. Epub 2017 Apr 27.
BACKGROUND: This study investigated the effects of amniotic membrane combined with adipose-derived stem cells or fetal fibroblasts on regenerating extensive burns in rats. METHODS: Third degree burns of 1100-1800 mm were induced on 32 Sprague-Dawley rats. Burned sites were excised and randomly covered with Vaseline gauze (control), human amniotic membrane (HAM), human fetal fibroblasts seeded on HAM (HAM-FF), or human adipose-derived stem cells seeded on HAM (HAM-ASC), and followed by wound closure and histological assessments. RESULTS: Wound closure rates of HAM-FF, HAM-ASC, HAM and control groups at seven and 14 days after the treatment were 42.2% and 81.9%, 41.9% and 81.7%, 33.5% and 74.2%, and 16.5% and 69.7%, respectively. Wounds of HAM-FF, HAM-ASC, HAM and control groups were closed on 40, 40, 50 and 60 days after the treatment, respectively (P < 0.05). Histological assessments revealed lower inflammatory cell infiltration in HAM-ASC and HAM-FF groups. CONCLUSIONS: Cell-based engineered skin substitutes seem to accelerate wound regeneration, especially within the first 14 days.
背景：本研究调查了羊膜联合脂肪来源干细胞或胎儿成纤维细胞对大鼠大面积烧伤再生的影响。 方法：对32只Sprague-Dawley大鼠造成1100 - 1800平方毫米的三度烧伤。切除烧伤部位，随机覆盖凡士林纱布（对照组）、人羊膜（HAM）、接种于HAM上的人胎儿成纤维细胞（HAM-FF）或接种于HAM上的人脂肪来源干细胞（HAM-ASC），随后进行伤口闭合及组织学评估。 结果：治疗后7天和14天，HAM-FF组、HAM-ASC组、HAM组和对照组的伤口闭合率分别为42.2%和81.9%、41.9%和81.7%、33.5%和74.2%、16.5%和69.7%。HAM-FF组、HAM-ASC组、HAM组和对照组的伤口分别在治疗后40天、40天、50天和60天闭合（P < 0.05）。组织学评估显示，HAM-ASC组和HAM-FF组的炎症细胞浸润较少。 结论：基于细胞的工程皮肤替代物似乎能加速伤口再生，尤其是在最初的14天内。
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