Taghiabadi Ehsan, Beiki Bahareh, Aghdami Nasser, Bajouri Amir
Department of Regenerative Biomedicine, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.
Skin and Stem Cell Research Center, Tehran University of Medical Sciences, Tehran, Iran.
Methods Mol Biol. 2019;1879:201-210. doi: 10.1007/7651_2018_173.
Application of cell-based skin substitutes has recently evolved as a novel treatment for hard-to-heal wounds. Here, we focus on the development of a novel skin substitute by seeding human adipose-derived stromal cells (ASCs) on acellular human amniotic membrane (HAM). This construction is probably associated with higher rates of host cell infiltration and implanted cell engraftment. ASCs are achieved by separation of stromal cells from lipoaspirates using collagenase digestion and acellular HAM was obtained by separation of outer membrane of the chorion and removing its epithelial cells.
基于细胞的皮肤替代物的应用近来已发展成为一种治疗难愈合伤口的新方法。在此，我们着重于通过将人脂肪来源的基质细胞（ASCs）接种于脱细胞人羊膜（HAM）上来开发一种新型皮肤替代物。这种构建可能与更高的宿主细胞浸润率和植入细胞的植入率相关。通过使用胶原酶消化从脂肪抽吸物中分离基质细胞来获得ASCs，通过分离绒毛膜的外膜并去除其上皮细胞来获得脱细胞HAM。
