Mahir A F, Laidding S R, Zainuddin A A, Mariana N, Syarif S, Islam A A, Massi M N, Cangara M H, Warsinggih W, Dososaputro I, Josh F, Bukhari A, Faruk M
Hasanuddin University, Makassar, Indonesia.
Airlangga University, Dr. Soetomo Hospital, Surabaya, Indonesia.
Ann Burns Fire Disasters. 2025 Jun 30;38(2):136-150. eCollection 2025 Jun.
Burn injuries cause a considerable number of fatalities in Indonesia annually. Platelet-rich plasma (PRP) in combination with stromal vascular fraction (SVF) is a stem cell-related treatment modality for deep dermal burn wound healing that utilizes growth factors such as the epidermal growth factor (EGF) and basic fibroblast growth factor (FGF2). This study aimed to assess the relationship between serum EGF and FGF2 levels and their impact on re-epithelialization and fibroblast histopathology during deep dermal burn wound healing using PRP and SVF. Wistar rats (n=64) were categorized into the following groups: PRP+SVF injection (Group A), topical PRP+SVF application (Group B), topical Vaseline application (Group C), or control (Group D). Treatments were evaluated on days 1, 4, 7, 10 and 14 post-treatment for the treatment groups and day 0 for the control group. The software SPSS 22 was used for the statistical analysis, along with the Shapiro-Wilk, independent t-test, Mann-Whitney, Kruskal-Wallis, one-way ANOVA, and Kendall's Tau-B tests. The results showed that Group A exhibited the highest FGF2 and EGF levels (6.62 and 8.10 ng/ml, respectively), closely followed by Group B (6.40 and 7.09 ng/ml, respectively). Group C had lower FGF2 levels than Group D and similar EGF levels. The PRP+SVF injection group displayed faster re-epithelialization and fibroblast proliferation. The PRP+SVF injections outperformed the topical application and Vaseline, with greater re-epithelialization and higher EGF levels. Although the injections showed comparable healing to the topical application, Vaseline produced proliferation differences during deep dermal burn wound recovery.
在印度尼西亚，烧伤每年导致大量死亡。富含血小板血浆（PRP）与基质血管成分（SVF）联合使用是一种用于深层真皮烧伤创面愈合的干细胞相关治疗方式，该方法利用了诸如表皮生长因子（EGF）和碱性成纤维细胞生长因子（FGF2）等生长因子。本研究旨在评估血清EGF和FGF2水平之间的关系，以及它们对使用PRP和SVF进行深层真皮烧伤创面愈合过程中再上皮化和成纤维细胞组织病理学的影响。将64只Wistar大鼠分为以下几组：PRP + SVF注射组（A组）、PRP + SVF局部应用组（B组）、局部应用凡士林组（C组）或对照组（D组）。对治疗组在治疗后第1、4、7、10和14天进行评估，对对照组在第0天进行评估。使用软件SPSS 22进行统计分析，同时采用Shapiro-Wilk检验、独立t检验、Mann-Whitney检验、Kruskal-Wallis检验、单因素方差分析和Kendall's Tau-B检验。结果显示，A组的FGF2和EGF水平最高（分别为6.62和8.10 ng/ml），紧随其后的是B组（分别为6.40和7.09 ng/ml）。C组的FGF2水平低于D组，EGF水平与D组相似。PRP + SVF注射组显示出更快的再上皮化和成纤维细胞增殖。PRP + SVF注射优于局部应用和凡士林，再上皮化程度更高，EGF水平也更高。虽然注射显示出与局部应用相当的愈合效果，但凡士林在深层真皮烧伤创面恢复过程中产生了增殖差异。
