A Molecular Basis for Selective Antagonist Destabilization of Dopamine D Receptor Quaternary Organization.

The dopamine D receptor (DR) is a molecular target for both first-generation and several recently-developed antipsychotic agents. Following stable expression of this mEGFP-tagged receptor, Spatial Intensity Distribution Analysis indicated that a substantial proportion of the receptor was present within dimeric/oligomeric complexes and that increased expression levels of the receptor favored a greater dimer to monomer ratio. Addition of the antipsychotics, spiperone or haloperidol, resulted in re-organization of DR quaternary structure to promote monomerization. This action was dependent on ligand concentration and reversed upon drug washout. By contrast, a number of other antagonists with high affinity at the DR, did not alter the dimer/monomer ratio. Molecular dynamics simulations following docking of each of the ligands into a model of the DR derived from the available atomic level structure, and comparisons to the receptor in the absence of ligand, were undertaken. They showed that, in contrast to the other antagonists, spiperone and haloperidol respectively increased the atomic distance between reference α carbon atoms of transmembrane domains IV and V and I and II, both of which provide key interfaces for DR dimerization. These results offer a molecular explanation for the distinctive ability of spiperone and haloperidol to disrupt DR dimerization.