Mamie Gao, Allen Zhong, Neil Patel, Chiraag Alur, Dinesh Vyas, Department of Surgery, Texas Tech University, Odessa, TX 79763, United States.
World J Gastroenterol. 2017 Apr 28;23(16):2819-2825. doi: 10.3748/wjg.v23.i16.2819.
RNA sequencing is the use of high throughput next generation sequencing technology to survey, characterize, and quantify the transcriptome of a genome. RNA sequencing has been used to analyze the pathogenesis of several malignancies such melanoma, lung cancer, and colorectal cancer. RNA sequencing can identify differential expression of genes (DEG's), mutated genes, fusion genes, and gene isoforms in disease states. RNA sequencing has been used in the investigation of several colorectal diseases such as colorectal cancer, inflammatory bowel disease (ulcerative colitis and Crohn's disease), and irritable bowel syndrome.
RNA 测序是利用高通量下一代测序技术来检测、描述和量化基因组的转录组。RNA 测序已被用于分析多种恶性肿瘤（如黑色素瘤、肺癌和结直肠癌）的发病机制。RNA 测序可以识别疾病状态下基因的差异表达（DEG）、突变基因、融合基因和基因异构体。RNA 测序已用于研究几种结直肠疾病，如结直肠癌、炎症性肠病（溃疡性结肠炎和克罗恩病）和肠易激综合征。
