Guo-Tao Yang, Ai-Qin Dong, Department of Third Neurology, Cangzhou Central Hospital, Cangzhou Clinical Medical School of Hebei Medical University, Cangzhou 061001, Hebei Province, China.
World J Gastroenterol. 2017 Apr 28;23(16):2940-2947. doi: 10.3748/wjg.v23.i16.2940.
To investigate the effects of nesfatin-1 on gastric function in obese rats.
The obese rat model was induced by a high-fat diet. The gastric emptying rate and gastric acid secretory capacity of the rats were determined after treatment with different drug concentrations of nesfatin-1 and administration routes. Based on this, the expression of H+/K+-ATPase was measured using RT-PCR and western blot to preliminarily explore the mechanism of gastric acid secretion changes.
Body weight, body length, and Lee's index of the rats significantly increased in the high-fat diet-induced obese rat model. Two hours after lateral intracerebroventricular injection of nesfatin-1, the gastric emptying rate and gastric acid secretory capacity of rats decreased. Four hours after injection, both were restored to normal levels. In addition, the expression of H+/K+-ATPase decreased and moved in line with changes in gastric acid secretory capacity. This experiment revealed that intracerebroventricular injection of nesfatin-1, rather than intravenous injection, could suppress gastric function in obese rats. Moreover, its effect on the gastric emptying and gastric acid secretory capacity of rats is dose-dependent within a certain period of time.
Through this research, we provide a theoretical basis for further studies on nesfatin-1, a potential anti-obesity drug.
研究内脂素-1 对肥胖大鼠胃功能的影响。
采用高脂饮食诱导肥胖大鼠模型，给予不同浓度的内脂素-1 及不同给药途径，检测大鼠胃排空率和胃酸分泌量，在此基础上通过 RT-PCR 和 Western blot 检测 H+/K+-ATPase 的表达，初步探讨胃酸分泌变化的机制。
高脂饮食诱导肥胖大鼠模型大鼠体重、体长和 Lee 指数明显增加。侧脑室注射内脂素-1 2 h 后，大鼠胃排空率和胃酸分泌量降低，4 h 后恢复正常水平。此外，H+/K+-ATPase 的表达降低，与胃酸分泌量的变化一致。本实验揭示了侧脑室注射内脂素-1 而非静脉注射可以抑制肥胖大鼠的胃功能，而且在一定时间内，其对大鼠胃排空和胃酸分泌的作用呈剂量依赖性。
通过这项研究，为进一步研究内脂素-1作为一种潜在的抗肥胖药物提供了理论依据。
