Faculty of Veterinary Medicine, Department of Reproduction and Clinic of Farm Animals, Wroclaw University of Environmental and Life Sciences, Pl. Grunwaldzki 49, 50-366 Wroclaw, Poland.
Faculty of Veterinary Medicine, Department of Immunology, Pathophysiology and Prevention Veterinary, Wroclaw University of Environmental and Life Sciences, ul. C.K. Norwida 31, 50-375 Wroclaw, Poland.
Biomed Res Int. 2017;2017:7279341. doi: 10.1155/2017/7279341. Epub 2017 Apr 24.
The objective of this study was to investigate the effect of L-carnitine (LC), hypotaurine (HT), and taurine (T) on the quality of frozen-thawed chicken semen. Pooled semen samples were divided into seven aliquots (control, 1 mM LC, 5 mM LC, 1 mM HT, 10 mM HT, 1 mM T, and 10 mM T) and subjected to cryopreservation. Postthaw sperm motility was determined by IVOS system and sperm characteristics were assessed with fluorochromes and flow cytometry. The highest sperm motility and the highest percentage of viable sperm were in the HT1 group ( < 0.01 and < 0.05) following cryopreservation. After thawing, we observed a higher percentage of sperm without apoptosis and membrane reorganization changes in the LC1 and T1 group when compared to the control ( < 0.05). There was a higher percentage of live sperm without lipid peroxidation (LPO) in all treatments ( < 0.01; < 0.05), when compared to the control group. The percentage of sperm with high mitochondrial potential significantly increased with LC1, T1, and T10 ( < 0.05). Supplementation of the diluent with LC1, LC5, and T1 significantly ( < 0.05) reduced DNA susceptibility to fragmentation, compared to the control and HT1 groups. These results indicate that the addition of examined antioxidants improves the quality of cryopreserved chicken semen.
本研究旨在探讨左旋肉碱（LC）、牛磺酸（HT）和牛磺酸（T）对冷冻-解冻鸡精液质量的影响。将混合精液样本分为 7 个等分试样（对照组、1mM LC、5mM LC、1mM HT、10mM HT、1mM T 和 10mM T），并进行冷冻保存。通过 IVOS 系统测定解冻后精子的运动能力，并使用荧光染料和流式细胞术评估精子特征。冷冻保存后，HT1 组的精子活力最高（<0.01），活精子比例最高（<0.05）。解冻后，与对照组相比，LC1 和 T1 组中无凋亡和膜重组变化的精子比例更高（<0.05）。与对照组相比，所有处理组的活精子中无脂质过氧化（LPO）的比例更高（<0.01；<0.05）。LC1、T1 和 T10 组的高线粒体潜能精子比例显著增加（<0.05）。与对照组和 HT1 组相比，稀释液中添加 LC1、LC5 和 T1 显著降低了精子 DNA 对碎片化的敏感性（<0.05）。这些结果表明，添加这些抗氧化剂可提高冷冻保存鸡精液的质量。
