Department of Zoology, Thiru. Vi. Ka. Government Arts College, Tiruvarur, Tamil Nadu, 610003, India.
Unit of Vector Control, Phytochemistry and Nanotechnology, Department of Zoology, Annamalai University, Annamalainagar, Tamil Nadu, 608 002, India.
Environ Sci Pollut Res Int. 2018 Apr;25(11):10317-10327. doi: 10.1007/s11356-017-9203-2. Epub 2017 May 18.
Recently, the biofabrication of metal nanoparticles has gained wide interest owing to its inherent features such as swift, simplicity, eco-friendliness, and cheaper costs. Different green-reducing agents led to the production of nanoparticles with varying toxicity on insects. In the current study, silver nanoparticles (AgNPs) were successfully synthesized using Habenaria plantaginea leaf extract. Ag nanoparticles were studied by UV-Vis spectroscopy (UV-Vis), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), atomic force microscopy (AFM), scanning electron microscopy (SEM) coupled with energy-dispersive spectroscopy (EDS), and transmission electron microscopy (TEM). H. plantaginea extract and AgNPs were tested for mosquito larvicidal activity on Anopheles stephensi, Aedes aegypti, Culex quinquefasciatus, An. subpictus, Ae. albopictus, and Cx. tritaeniorhynchus. LC values were 102.51, 111.99, 123.47, 123.96, 136.56, 149.42 μg/ml and 12.23, 13.38, 14.78, 14.37, 15.39, 16.89 μg/ml, respectively. Moreover, H. plantaginea aqueous extract and AgNPs were tested against the non-target species Anisops bouvieri, Diplonychus indicus, Poecilia reticulata, and Gambusia affinis obtaining LC values ranging from 831.82 to 36,212.67 μg/ml. Overall, this study showed the effectiveness of H. plantaginea-fabricated nanoparticles on a wide range of important mosquito vectors, highlighting their scarce toxicity on four natural enemies predating mosquito larvae and pupae.
最近，由于其快速、简单、环保和成本低廉等固有特点，金属纳米粒子的生物制造引起了广泛的关注。不同的绿色还原剂导致了具有不同昆虫毒性的纳米粒子的产生。在本研究中，成功地使用兰科植物绶草的叶子提取物合成了银纳米粒子（AgNPs）。通过紫外可见光谱（UV-Vis）、X 射线衍射（XRD）、傅里叶变换红外光谱（FTIR）、原子力显微镜（AFM）、扫描电子显微镜（SEM）耦合能谱（EDS）和透射电子显微镜（TEM）对 Ag 纳米粒子进行了研究。对绶草提取物和 AgNPs 进行了对按蚊、埃及伊蚊、致倦库蚊、淡色库蚊、白纹伊蚊和三带喙库蚊的杀蚊幼虫活性测试。LC 值分别为 102.51、111.99、123.47、123.96、136.56 和 149.42μg/ml，12.23、13.38、14.78、14.37、15.39 和 16.89μg/ml。此外，对非靶标物种中华按蚊、印度食蚊鱼、孔雀鱼和食蚊鱼进行了绶草水提物和 AgNPs 的测试，LC 值范围为 831.82 至 36212.67μg/ml。总的来说，这项研究表明了绶草制造的纳米粒子对广泛的重要蚊子传播媒介的有效性，突出了它们对四种捕食蚊子幼虫和蛹的天然捕食者的毒性很小。
