Schulz-Key H
Institute for Tropical Medicine, University of Tübingen, FRG.
Trop Med Parasitol. 1988 Dec;39 Suppl 4:423-40.
The collagenase technique developed more than twelve years ago has successfully been applied to isolate living and intact O. volvulus from excised onchocercomata for various biological, morphological, biochemical, immunological and in vitro studies. The experience gained with this technique and its modifications is critically reviewed giving practical advice for the selection, preservation and treatment of nodules for different purposes, especially for the evaluation of antifilarial drug effects. The composition of the worm load, the morphology of adult worms found in untreated patients, signs of degeneration and ageing of worms are described to show the broad morphological spectrum of the worm population in endemic areas to avoid false conclusions in drug trials. When these parameters are carefully considered, the collagenase technique provides a reliable method for examination of onchocercomata. An immediate answer can be obtained concerning the viability of the parasite. The qualitative and quantitative assessment of reproduction (embryogram) can give further valuable information on any changes in the reproductive dynamics and provide a sensitive indicator of any disturbances in the adult worms which may explain the early or late reinvasion of microfilariae into the skin after treatment with microfilaricidal drugs.
十二多年前开发的胶原酶技术已成功应用于从切除的盘尾丝虫瘤中分离出活的和完整的旋盘尾丝虫，用于各种生物学、形态学、生物化学、免疫学和体外研究。对该技术及其改进所获得的经验进行了批判性回顾，针对不同目的的结节选择、保存和处理提供了实用建议，特别是用于评估抗丝虫药物的效果。描述了虫负荷的组成、未治疗患者体内发现的成虫形态、虫体退化和衰老的迹象，以展示流行地区虫群广泛的形态谱，避免在药物试验中得出错误结论。当仔细考虑这些参数时，胶原酶技术为盘尾丝虫瘤的检查提供了一种可靠的方法。可以立即获得有关寄生虫活力的答案。对生殖（胚胎图）的定性和定量评估可以提供有关生殖动态变化的进一步有价值信息，并为成虫的任何干扰提供敏感指标，这可能解释了用杀微丝蚴药物治疗后微丝蚴早期或晚期重新侵入皮肤的情况。
