a National Institute of Plant Genome Research (NIPGR) , New Delhi , India.
b Department of Plant Molecular Biology , University of Delhi South Campus , New Delhi , India.
Crit Rev Biochem Mol Biol. 2017 Oct;52(5):475-502. doi: 10.1080/10409238.2017.1325830. Epub 2017 May 19.
Mediator is a multi-subunit protein complex which is involved in transcriptional regulation in yeast and other eukaryotes. As a co-activator, it connects information from transcriptional activators/repressors to transcriptional machinery including RNA polymerase II and general transcription factors. It is not only involved in transcription initiation but also has important roles to play in transcription elongation and termination. Functional attributes of different Mediator subunits have been largely defined in yeast and mammalian systems earlier, while such studies in plants have gained momentum recently. Mediator regulates various processes related to plant development and is also involved in biotic and abiotic stress response. Thus, plant Mediator, like yeast and mammalian Mediator complex, is indispensable for plant growth and survival. Interaction of its multiple subunits with other regulatory proteins and their ectopic expression or knockdown in model plant like Arabidopsis and certain crop plants are paving the way to biochemical analysis and unravel molecular mechanisms of action of Mediator in plants.
中介体是一种多亚基蛋白复合物，参与酵母和其他真核生物的转录调控。作为一个共激活因子，它将来自转录激活物/阻遏物的信息连接到包括 RNA 聚合酶 II 和一般转录因子在内的转录机制上。它不仅参与转录起始，而且在转录延伸和终止中也发挥着重要作用。不同中介体亚基的功能特性在早期的酵母和哺乳动物系统中已被广泛定义，而最近在植物中的研究也取得了进展。中介体调节与植物发育相关的各种过程，并且还参与生物和非生物胁迫反应。因此，植物中介体与酵母和哺乳动物中介体复合物一样，对植物的生长和存活是不可或缺的。其多个亚基与其他调节蛋白的相互作用，以及在拟南芥和某些作物等模式植物中的异位表达或敲低，为生化分析和揭示植物中介体的作用分子机制铺平了道路。
