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TOPLESS 核心抑制因子的抑制作用需要与核心中介复合物结合。

Repression by the TOPLESS corepressor requires association with the core mediator complex.

机构信息

Department of Biology, University of Washington, Seattle, United States.

Department of Pharmacology, Seattle, United States.

出版信息

Elife. 2021 Jun 2;10:e66739. doi: 10.7554/eLife.66739.

DOI:10.7554/eLife.66739
PMID:34075876
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8203292/
Abstract

The plant corepressor TOPLESS (TPL) is recruited to a large number of loci that are selectively induced in response to developmental or environmental cues, yet the mechanisms by which it inhibits expression in the absence of these stimuli are poorly understood. Previously, we had used the N-terminus of TPL to enable repression of a synthetic auxin response circuit in (yeast). Here, we leveraged the yeast system to interrogate the relationship between TPL structure and function, specifically scanning for repression domains. We identified a potent repression domain in Helix 8 located within the CRA domain, which directly interacted with the Mediator middle module subunits Med21 and Med10. Interactions between TPL and Mediator were required to fully repress transcription in both yeast and plants. In contrast, we found that multimer formation, a conserved feature of many corepressors, had minimal influence on the repression strength of TPL.

摘要

植物核心抑制因子 TOPLESS（TPL）被招募到大量的基因座，这些基因座在发育或环境信号的刺激下选择性地被诱导，但它在没有这些刺激的情况下抑制表达的机制尚不清楚。以前，我们使用 TPL 的 N 端来抑制酵母中合成的生长素反应回路的表达。在这里，我们利用酵母系统来探究 TPL 结构和功能之间的关系，特别是扫描抑制结构域。我们在位于 CRA 结构域内的螺旋 8 中发现了一个有效的抑制结构域，它直接与 Mediator 中间模块亚基 Med21 和 Med10 相互作用。TPL 和 Mediator 之间的相互作用对于在酵母和植物中完全抑制转录都是必需的。相比之下，我们发现多聚体形成，许多核心抑制因子的保守特征，对 TPL 的抑制强度影响很小。

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